Preparation and characterization of long alkyl chain methacrylate-based monolithic column for capillary chromatography
Preparation and characterization of long alkyl chain methacrylate-based monolithic column for capillary chromatography
复制标题
DOI：
10.1016/j.jbbm.2006.08.009
复制
发表时间：
2007-02-23
影响因子：
--
通讯作者：
Taylor, Mark Robert
中科院分区：
文献类型：
--
作者：
Jiang, Zhengjin;Smith, Norman William;Taylor, Mark Robert
本文报道了一种用于毛细管液相色谱固定相的长链甲基丙烯酸酯整体材料的制备。在用甲基丙烯酸3-（三甲氧基甲硅烷基）丙酯（γ-MAPS）使毛细管表面失活之后，在引发剂AIBN和包括异戊醇和1，4-丁二醇的致孔剂的混合物的存在下，通过甲基丙烯酸硬脂酯（SMA）与乙二醇二甲基丙烯酸酯（EDMA）的共聚形成整料。在100 μ m W.毛细管和聚合混合物的组成在SMA/EDMA的比率、致孔剂组成和致孔剂与单体的比率方面进行优化。随着致孔剂重量分数降低，微球变得更小，并且如预期的，总孔隙率降低。为了确定这种材料的可用性，通过以不同的线性流速将水泵送通过柱来测量柱渗透性K。用这种毛细管对弱酸、中性和碱性混合物进行了分离，取得了良好的效果。(c)2006 Elsevier B.V.保留所有权利。
This paper describes the fabrication of long alkyl chain methacrylate monolithic materials for using as stationary phases in capillary liquid chromatography. Following deactivation of the capillary surface with 3-(trimethoxysilyl)propyl methacrylate (gamma-MAPS), monoliths were formed by co-polymerisation of stearyl methacrylate (SMA) with ethylene glycol dimethacrylate (EDMA) in the presence of the initiator AIBN and a mixture of porogens including iso-amyl alcohol and 1,4-butanediol. The monoliths were prepared in 100 pm W. capillaries and the composition of the polymerisation mixtures were optimised in terms of the ratio of SMA/EDMA, the porogen composition and ratio of porogen to monomers. As the porogen weight fraction decreased, the microglobules became smaller and as expected, the total porosity decreased. In order to determine the usability of such materials, the column permeability K was measured by pumping water through the columns at different linear flow rates. Good results were obtained when these capillaries were used to separate mixtures of weak acids, neutral and basic compounds. (c) 2006 Elsevier B.V. All rights reserved.