Neuropilin-1 is not a marker of human Foxp3+ Treg
Neuropilin-1 is not a marker of human Foxp3+ Treg
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DOI：
10.1002/eji.200839040
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发表时间：
2009-06-01
影响因子：
5.4
通讯作者：
Hermine, Olivier
中科院分区：
文献类型：
--
作者：
Milpied, Pierre;Renand, Amedee;Hermine, Olivier
Treg是一种免疫细胞，在免疫反应的调节中起着关键作用。尽管转录因子Foxp3被广泛认为是Treg的标准标记，但需要特定的表面标记来更好地描述这些细胞并破译它们的作用机制。NRP-1是一种主要参与神经系统的膜蛋白，被认为是小鼠Treg的特异性标志物，但其在人类Treg中的表达尚未得到严格的研究。在这里，我们表明，与小鼠Treg不同，无论它们的来源是血液、胸腺、脾、淋巴还是扁桃体，人类Foxp3(+)Treg并不特异性地表达NRP-1。然而，在人的次级淋巴器官中可以检测到大量的Foxp3(-)NRP-1(+)T细胞，在体外激活后，NRP-1在外周血T淋巴细胞上被诱导表达。结论：NRP-1不能作为人Treg的特异性标志物，但在体内外可能是一种新的人T细胞活化标志物。
Treg are immune cells that play a critical role in the regulation of the immune response. Although the transcription factor Foxp3 is widely accepted as the standard marker of Treg, specific surface markers are needed to better characterize these cells and decipher their mechanisms of action. Neuropilin-1 (Nrp-1), a membrane protein primarily involved in the nervous system, was identified as a specific marker of murine Treg, but its expression has not been rigorously investigated in human Treg. Here we show that in contrast to murine Treg and regardless of their origins (blood, thymus, spleen, lymph node or tonsil), human Foxp3(+) Treg do not specifically express Nrp-1. However, a population of Foxp3(-) Nrp-1(+) T cells can be detected in human secondary lymphoid organs, and Nrp-1 expression is induced on peripheral blood T lymphocytes upon in vitro activation. We conclude that Nrp-1 cannot be used as a specific marker of human Treg, but might represent a novel activation marker of human T cells both in vitro and in vivo.