Use of DNA arrays to study transcriptional responses to antimycobacterial compounds.
Use of DNA arrays to study transcriptional responses to antimycobacterial compounds.
复制标题
使用 DNA 阵列研究抗分枝杆菌化合物的转录反应。
作者：
S. Waddell;P. Butcher
对结核分枝杆菌经抗分枝杆菌化合物处理后的转录谱进行分析，提高了我们对分枝杆菌对抗生素应激反应方式的理解，并揭示了不同抗分枝杆菌化合物类的作用模式。药物诱导的变化的RNA图谱已经成为抗菌药物开发过程中从靶点阐明、识别靶点漂移到最终揭示耐药机制的多个阶段的重要工具。结核分枝杆菌对抗分枝杆菌化合物的转录反应可以通过分离、与其他化合物类别的比较或在药物敏感和耐药的分枝杆菌分离株之间进行确定。确认分枝杆菌在添加抗菌化合物时的生长状态以及该化合物对分枝杆菌生长的影响的额外信息，对于解释获得的转录特征是必不可少的。本章描述了提取具有代表性的分枝杆菌总RNA所需的方法，随后将该RNA与结核分枝杆菌复合微阵列杂交，以及用来解释产生的转录数据的分析策略。
Analysis of the transcriptional profiles of Mycobacterium tuberculosis after treatment with antimycobacterial compounds has improved our understanding of the ways mycobacteria respond to antibiotic stress, and revealed new insights into the mode of action of different antimycobacterial compound classes. RNA profiling of drug-induced changes has become an important tool in multiple stages of the antibacterial drug development process from target elucidation, to identifying target drift, and ultimately to revealing drug resistance mechanisms. The transcriptional response of M. tuberculosis to antimycobacterial compounds may be determined in isolation, in comparison with other compound classes, or between drug-sensitive and drug-resistant mycobacterial isolates. Additional information confirming the growth state of mycobacteria on addition of the antibacterial compound, and the effect that this compound has on mycobacterial growth, is essential for interpreting the transcriptional signatures acquired. This chapter describes the methods required for the extraction of representative total mycobacterial RNA, the subsequent hybridisation of this RNA to an M. tuberculosis complex microarray, and the analysis strategies employed to interpret the transcriptional data generated.
DOI：
10.1073/pnas.96.22.12833
发表时间：
1999-10-26
影响因子：
11.1
作者：
Wilson, W;DeRisi, J;Schoolnik, GK
通讯作者：
Schoolnik, GK
影响因子：
3.2
作者：
Fu, LM
通讯作者：
Fu, LM
影响因子：
3.2
作者：
Fu, Li M.;Shinnick, Thomas M.
通讯作者：
Shinnick, Thomas M.