Deletion/insertion polymorphisms of the prion protein gene (PRNP) in gayal (Bos frontalis)
Deletion/insertion polymorphisms of the prion protein gene (PRNP) in gayal (Bos frontalis)
复制标题
盖亚尔（Bos frontalis）朊病毒蛋白基因（PRNP）的缺失/插入多态性
DOI：
10.1007/s12041-018-1005-x
复制
发表时间：
2018-12-01
影响因子：
1.5
通讯作者：
Xi, Dongmei
中科院分区：
文献类型：
--
作者：
Memon, Sameeullah;Li, Guozhi;Xi, Dongmei
牛对致死性疾病牛海绵状脑病(BSE)的抗性与PrNP基因启动子23bpIndel多态和内含子1 12bpindel相关。Gayal(Bos Frontalis)是一种重要的半野生牛种，受到极大的保护，但到目前为止，还没有在Gayal中对这些indel多态进行评估。因此，我们收集了225份同性恋者的样本，并对该PRNP基因的两个区域的遗传多态性进行了评估。结果显示，这些插入位点的等位基因频率较高：23bP和12bpINDELs的等位基因频率分别为0.909和0.667，均具有显著的基因型频率(2：9.81；23bp和2：43.56；12bp)。同时，单倍型数据显示，在PRNP基因的两个区域都存在极低的缺失(0.01)。我们将这些数据与德国和瑞士两个欧洲国家报道的健康和感染疯牛病的牛(Bos Taurus)品种的数据进行了比较，发现感染疯牛病的牛和健康牛之间存在显著差异(P0.166667em0.166667em“0.001)。此外，我们的数据还与以前关于BSE的报告进行了广泛的比较，并观察到了非常显著的结果(P0.166667em0.166667e0.001)。这一结果表明同性恋者对疯牛病的遗传易感性可以忽略不计。据我们所知，这项研究是首次全面破译中国属半野生物种--长颈鹿的23和12个碱基的重复序列多态。
Resistance to fatal disease bovine spongiform encephalopathy (BSE), due to misfolded prion protein in cattle, is associated with a 23-bp indel polymorphism in the putative promoter and a 12-bp indel in intron 1 of the PRNP gene. Gayal (Bos frontalis) is an important semiwild bovid species and of great conservation concern, but till today these indel polymorphisms have not been evaluated in gayals. Therefore, we collected 225 samples of gayals and evaluated the genetic indel polymorphism in the two regions of this PRNP gene. The results revealed high allelic frequencies of insertions at these indel sites: 0.909 and 0.667 for, respectively, the 23 bp and 12 bp indels, both also with significant genotype frequencies (2: 9.81; 23 bp and 2: 43.56; 12 bp). At the same time, the haplotype data showed indel polymorphisms with extremely low deletion (0.01) in both regions of the PRNP gene. We compared these data with those reported for healthy and BSE-affected cattle (Bos taurus) breeds from two European countries, Germany and Switzerland, and significant difference (P0.166667em0.166667em"0.001) was observed between BSE-affected as well as the healthy cattle. Further, our data were also extensively compared with previous reports on BSE and highly significant (P0.166667em0.166667e0.001) outcomes were observed. This result suggested negligible genetic susceptibility to BSE in gayals. To the best of our knowledge, this study is the first comprehensive deciphering information about the PRNP indel polymorphisms of 23 bp and 12 bp in gayals, a semiwild species of China.