Keishi-ka-kei-to, a traditional Chinese herbal medicine, inhibits pulmonary metastasis of B16 melanoma.
Keishi-ka-kei-to, a traditional Chinese herbal medicine, inhibits pulmonary metastasis of B16 melanoma.
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Keishi-ka-kei-to 是一种传统中草药，可抑制 B16 黑色素瘤的肺转移。
作者：
F. Suzuki;M. Kobayashi;Y. Komatsu;Akihiko Kato;R. Pollard
桂皮、白芍、酸枣仁、生姜、甘草等五种药用植物的粗提物组成的中药可抑制B16F10黑色素瘤细胞移植小鼠的实验性肺转移。当接种1×10(5)细胞/只黑色素瘤细胞的小鼠肺内产生136~145个转移集落时，经口给予80~320 mg/kg的凯时可得不到15个转移集落。混合物中最有效的成分是从生姜提取物中提取的6-姜酚。6-姜辣素对B16F10黑色素瘤细胞体外生长无影响，其抗肿瘤活性是通过宿主的抗肿瘤免疫功能表现出来的。将该化合物处理的小鼠脾CD8+T细胞过继转移到荷B16F10黑色素瘤细胞的小鼠体内，显示出抑制肺转移的活性。提示凯时可通过刺激CD8+T细胞抑制荷B16F10黑色素瘤小鼠的肺转移。
Keishi-ka-kei-to, a traditional Chinese herbal medicine composed of a mixture of crude extracts from five medicinal plants (Cinnamomi cortex, Paeoniae radix, Zizyphi fructus, Zingiberis rhizoma and Glycyrrhizae radix), inhibited experimental pulmonary metastasis in mice implanted with B16F10 melanoma cells. When 136 to 145 metastatic colonies were produced in lungs of mice inoculated with 1 x 10(5) cells/mouse of melanoma cells, less than 15 metastatic colonies were demonstrated when these tumor-inoculated mice were treated orally with 80 to 320 mg/kg doses of Keishi-ka-kei-to. The most active component in the mixture was shown to be 6-gingerol, derived from the Zingiberis rhizoma extract. The antimetastatic activity of 6-gingerol was expressed through the host's antitumor immune functions, as the growth of B16F10 melanoma cells was not affected by this substance in vitro. The splenic CD8+ T cells from mice treated with the compound showed inhibitory activities on pulmonary metastasis when these T cells were adoptively transferred to mice bearing B16F10 melanoma cells. These results may suggest that Keishi-ka-kei-to inhibits pulmonary metastasis in mice bearing B16F10 melanoma cells through the stimulation of CD8+ T cells.