I-Optimal design of poly(lactic-co-glycolic) acid/hydroxyapatite three-dimensional scaffolds produced by thermally induced phase separation
I-Optimal design of poly(lactic-co-glycolic) acid/hydroxyapatite three-dimensional scaffolds produced by thermally induced phase separation
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DOI：
10.1002/pen.25094
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发表时间：
2019-06-01
影响因子：
3.2
通讯作者：
Yousefi, Azizeh-Mitra
中科院分区：
文献类型：
--
作者：
Liu, Junyi;Zhang, Jing;Yousefi, Azizeh-Mitra
在骨组织工程中，三维（3D）支架通常被设计为具有足够的模量，同时考虑到细胞接种和组织生长对高度多孔网络的要求。本文介绍了由聚乳酸-羟基乙酸共聚物（PLGA）和纳米羟基磷灰石（nHA）组成的三维支架的设计优化，通过热诱导相分离（TIPS）制备。以1摄氏度/分钟的速率缓慢冷却能够实现均匀的温度并产生具有相对均匀孔径的多孔支架。使用具有18次实验运行的I-最优实验设计（DoE）将四个响应（支架厚度、密度、孔隙率和模量）与三个实验因素相关联，即TIPS温度（-20、-10和0 ℃）、PLGA浓度（7%、10%和13%w/v）和nHA含量（0%、15%和30%w/w）。使用JMP（R）软件的响应面分析预测温度为-18.3 ℃，PLGA浓度为10.3%w/v，nHA含量为30%w/w，以实现3 mm的厚度，83%的孔隙率和类似于4 MPa的模量。使用预测因子水平制备的验证支架组的厚度为3.05 +/- 0.37 mm，孔隙率为86.8 +/-0.9%，模量为3.57 +/- 2.28 MPa。Polym.工程索引，59：1146-1157 2019. (c)2019年塑料工程师协会
In bone tissue engineering, three-dimensional (3D) scaffolds are often designed to have adequate modulus while taking into consideration the requirement for a highly porous network for cell seeding and tissue growth. This article presents the design optimization of 3D scaffolds made of poly(lactic-co-glycolic) acid (PLGA) and nanohydroxyapatite (nHA), produced by thermally induced phase separation (TIPS). Slow cooling at a rate of 1 degrees C/min enabled a uniform temperature and produced porous scaffolds with a relatively uniform pore size. An I-optimal design of experiments (DoE) with 18 experimental runs was used to relate four responses (scaffold thickness, density, porosity, and modulus) to three experimental factors, namely the TIPS temperature (-20, -10, and 0 degrees C), PLGA concentration (7%, 10%, and 13% w/v), and nHA content (0%, 15%, and 30% w/w). The response surface analysis using JMP (R) software predicted a temperature of -18.3 degrees C, a PLGA concentration of 10.3% w/v, and a nHA content of 30% w/w to achieve a thickness of 3 mm, a porosity of 83%, and a modulus of similar to 4 MPa. The set of validation scaffolds prepared using the predicted factor levels had a thickness of 3.05 +/- 0.37 mm, a porosity of 86.8 +/- 0.9%, and a modulus of 3.57 +/- 2.28 MPa. POLYM. ENG. SCI., 59:1146-1157 2019. (c) 2019 Society of Plastics Engineers