TARGETED INSERTION OF A VARIABLE REGION GENE INTO THE IMMUNOGLOBULIN HEAVY-CHAIN LOCUS
TARGETED INSERTION OF A VARIABLE REGION GENE INTO THE IMMUNOGLOBULIN HEAVY-CHAIN LOCUS
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DOI：
10.1126/science.8235657
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发表时间：
1993-11-19
期刊：
影响因子：
56.9
通讯作者：
RAJEWSKY, K
中科院分区：
文献类型：
--
作者：
TAKI, S;MEIERING, M;RAJEWSKY, K
已产生突变小鼠品系，其中重排的免疫球蛋白重 (H) 链可变 (V) 区基因被置于重链基因座的天然位置，取代了 J(H) 元件。在纯合突变小鼠中，基本上脾脏中的所有 B 细胞都在其抗体中表达转基因 V(H) 区。转基因相对于恒定区基因的正确位置使其能够参与同种型转换并经历体细胞超突变。通过基因靶向以这种方式产生的免疫球蛋白转基因小鼠应该被证明对于探索免疫调节机制是有用的。
A mutant mouse strain has been generated in which a rearranged immunoglobulin heavy (H) chain variable (V) region gene is placed into the heavy chain locus in its natural position, replacing the J(H) elements. In homozygous mutant mice, essentially all B cells in the spleen express the transgenic V(H) region in their antibodies. The proper location of the transgene relative to the constant region genes allows it to participate in isotype switching and undergo somatic hypermutation. Immunoglobulin transgenic mice generated in this fashion by gene targeting should prove useful for the exploration of immunoregulatory mechanisms.