Polo-like kinase-1 triggers histone phosphorylation by Haspin in mitosis
Polo-like kinase-1 triggers histone phosphorylation by Haspin in mitosis
复制标题
Polo 样激酶 1 通过 Haspin 在有丝分裂中触发组蛋白磷酸化
DOI：
10.1002/embr.201338080
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发表时间：
2014
期刊：
影响因子：
7.7
通讯作者：
Higgins Jonathan M. G.
中科院分区：
文献类型：
--
作者：
Zhou Linli;Tian Xiaoying;Zhu Cailei;Wang Fangwei;Higgins Jonathan M. G.
组蛋白修饰协调有丝分裂中关键调节因子的染色质定位。例如，Haspin对组蛋白H3苏氨酸-3 （H3T3ph）的有丝分裂磷酸化为染色体乘客复合体（CPC）创造了一个结合位点。然而，在细胞周期中，这些组蛋白修饰如何在时空上受到控制尚不清楚。在这里，我们发现Plk1以cdk1磷酸化依赖的方式与Haspin结合。降低Plk1活性会降低Haspin的磷酸化并抑制H3T3ph，特别是在前期，这表明Plk1是有丝分裂早期Haspin初始激活所必需的。这些研究表明Plk1可以正向调节有丝分裂过程中CPC的募集。
Histone modifications coordinate the chromatin localization of key regulatory factors in mitosis. For example, mitotic phosphorylation of Histone H3 threonine-3 (H3T3ph) by Haspin creates a binding site for the chromosomal passenger complex (CPC). However, how these histone modifications are spatiotemporally controlled during the cell cycle is unclear. Here we show that Plk1 binds to Haspin in a Cdk1-phosphorylation-dependent manner. Reducing Plk1 activity decreases the phosphorylation of Haspin and inhibits H3T3ph, particularly in prophase, suggesting that Plk1 is required for initial activation of Haspin in early mitosis. These studies demonstrate that Plk1 can positively regulate CPC recruitment in mitosis.