UV photofunctionalization promotes nano-biomimetic apatite deposition on titanium.
UV photofunctionalization promotes nano-biomimetic apatite deposition on titanium.
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DOI：
10.2147/ijn.s95249
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发表时间：
2016
影响因子：
8
通讯作者：
Ogawa T
中科院分区：
文献类型：
--
作者：
Saita M;Ikeda T;Yamada M;Kimoto K;Lee MC;Ogawa T
尽管仿生磷灰石涂层是为钛提供骨传导性的一种有前途的方法，但沉积的效率和质量往往很差。大多数钛植入物具有微米级表面形态，在保留微米形态的同时添加纳米级特征可能会提供进一步的生物学益处。在这里，我们研究了钛的紫外 (UV) 光处理或光功能化对钛上仿生磷灰石沉积的功效及其生物性能的影响。采用硫酸酸蚀法制备微粗糙钛盘。将具有或不具有光功能化（紫外线照射 20 分钟）的微粗糙圆盘浸入模拟体液 (SBF) 中 1 或 5 天。光功能化钛圆盘具有超亲水性，浸入SBF 中时不会形成表面气泡，而非光功能化钛圆盘具有疏水性，在浸入过程中大部分被气泡覆盖。 SBF 浸泡 1 天后，通过 X 射线衍射在光功能化钛上观察到与磷灰石相关的信号，这与对照钛浸泡 5 天后观察到的信号相当。扫描电子显微镜显示在微粗糙结构的山谷和倾斜处有结节状磷灰石沉积，而不影响现有的微观结构。微粗糙化钛和磷灰石沉积钛表面具有相似的粗糙度值。骨髓源性成骨细胞的附着、铺展、沉降、增殖和碱性磷酸盐活性在磷灰石涂层钛上的光功能化得到促进。钛的紫外光功能化能够更快地沉积纳米级仿生磷灰石，与类似制备的未经光功能化的磷灰石沉积钛相比，其生物性能得到改善。光功能化辅助仿生磷灰石沉积可能是一种有效增强微粗糙钛表面而不改变其微观形态的新方法。
Although biomimetic apatite coating is a promising way to provide titanium with osteoconductivity, the efficiency and quality of deposition is often poor. Most titanium implants have microscale surface morphology, and an addition of nanoscale features while preserving the micromorphology may provide further biological benefit. Here, we examined the effect of ultraviolet (UV) light treatment of titanium, or photofunctionalization, on the efficacy of biomimetic apatite deposition on titanium and its biological capability. Micro-roughed titanium disks were prepared by acid-etching with sulfuric acid. Micro-roughened disks with or without photofunctionalization (20-minute exposure to UV light) were immersed in simulated body fluid (SBF) for 1 or 5 days. Photofunctionalized titanium disks were superhydrophilic and did not form surface air bubbles when immersed in SBF, whereas non-photofunctionalized disks were hydrophobic and largely covered with air bubbles during immersion. An apatite-related signal was observed by X-ray diffraction on photofunctionalized titanium after 1 day of SBF immersion, which was equivalent to the one observed after 5 days of immersion of control titanium. Scanning electron microscopy revealed nodular apatite deposition in the valleys and at the inclines of micro-roughened structures without affecting the existing micro-configuration. Micro-roughened titanium and apatite-deposited titanium surfaces had similar roughness values. The attachment, spreading, settling, proliferation, and alkaline phosphate activity of bone marrow-derived osteoblasts were promoted on apatite-coated titanium with photofunctionalization. UV-photofunctionalization of titanium enabled faster deposition of nanoscale biomimetic apatite, resulting in the improved biological capability compared to the similarly prepared apatite-deposited titanium without photofunctionalization. Photofunctionalization-assisted biomimetic apatite deposition may be a novel method to effectively enhance micro-roughened titanium surfaces without altering their microscale morphology.