Potential use of characterised hyper-colonising strain(s) of Campylobacter jejuni to reduce circulation of environmental strains in commercial poultry
Potential use of characterised hyper-colonising strain(s) of Campylobacter jejuni to reduce circulation of environmental strains in commercial poultry
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DOI：
10.1016/j.vetmic.2008.09.055
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发表时间：
2009-03-02
影响因子：
3.3
通讯作者：
Korolik, Victoria
中科院分区：
文献类型：
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作者：
Calderon-Gomez, Lirio I.;Hartley, Lauren E.;Korolik, Victoria
62个持续定殖空肠弯曲杆菌菌株进行了测试，其能力占主导地位的鸡胃肠道的殖民在相互竞争，导致选择占主导地位的或“超定殖”的弯曲杆菌菌株，这是能够取代其他在鸡肠道。其中一种菌株被证明是鸡的超高效定殖者，因为它能够取代其他定殖菌株，并在56天肉鸡生产周期的持续时间内保持自身在鸡肠道中。一旦殖民建立，这种超殖民C。空肠菌株331不能被其他定殖或超定殖菌株所取代。我们提出，一个定义的，超定殖菌株，或具有类似表型的定义菌株的鸡尾酒，可以形成生物控制未知/未表征的弯曲杆菌菌株的基础，从环境中不断殖民鸡群。为了验证这种方法，进行了三种不同的鸡感染试验。这些试验表明，空肠弯曲杆菌的优势菌株能够在肉鸡中持续定植，并且在鸡的整个生命周期内定植，而与接种日期和饲料中用于控制其他病原微生物的抗菌剂无关。此外，我们已经表明，无论接种时间和类型如何，生物控制菌株都能够在56天肉鸡生产周期的各个点取代其他定殖菌株。该菌株在用其他菌株攻毒后（有和无再攻毒）也能够重新建立自身。这项工作代表了一个“原则证明”，一个定义的C。空肠菌株可用于生物学控制商业家禽群中未表征的环境菌株的循环。(C)2008 Elsevier B. V.保留所有权利。
Sixty-two persistently colonising Campylobacter jejuni strains were tested for their ability to dominate colonisation of the chicken gastrointestinal tract in competition with each other leading to selection of dominant or "hyper-colonising" Campylobacter strains, which are able to displace others in the chicken intestinal tract. One such strain was shown to be a hyper-efficient coloniser of chickens, as it was able to displace other colonising strains, as well as maintain itself in the chicken intestinal tract for the duration of the 56-day broiler production cycle. Once colonisation was established, this hyper-colonising C. jejuni strain, 331, could not be displaced by other colonising or hyper-colonising strains. We proposed that a defined, hyper-colonising strain, or a cocktail of defined strains with a similar phenotype, could form the basis for biological control of unknown/uncharacterised Campylobacter strains from the environment that continuously colonise chicken flocks. To validate this approach, three different chicken infection trials were carried Out. These trials demonstrated that the dominant strain of C, jejuni was able to colonise broiler chickens consistently and for the entire life of the birds irrespective of the day of inoculation and antimicrobial agents used in the feed to control other pathogenic micro-organisms. In addition, we have shown that the bio-control strain was able to replace other colonising strains at various points of a 56-day broiler production cycle irrespective of time and type of inoculation. This strain was also capable of re-establishing itself following the challenge with other strains, with and without re-challenge. This work represents a "proof of principle" that a defined C. jejuni strain could be used to biologically control circulation of uncharacterised environmental strains in commercial poultry flocks. (C) 2008 Elsevier B.V. All rights reserved.