Intervertebral disc cell death is dependent on the magnitude and duration of spinal loading
Intervertebral disc cell death is dependent on the magnitude and duration of spinal loading
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DOI：
10.1097/00007632-200006150-00005
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发表时间：
2000-06-15
期刊：
影响因子：
3
通讯作者：
Chin, JR
中科院分区：
文献类型：
--
作者：
Lotz, JC;Chin, JR
研究设计.静态压缩应力对椎间盘毒性后果的体内研究。确定椎间盘细胞死亡是否与脊柱压缩负荷的大小和持续时间相关。体内静态压缩已被证明可诱导细胞死亡。反过来，细胞死亡与人类椎间盘退变有关。目前还没有结合生物力学和生物学因素的联合收割机的椎间盘耐受标准，尽管两者都涉及加速变性的病例。用外部压缩装置在体内加载小鼠尾盘。以两个量级之一（0.4和0.8 MPa）施加压缩应力7天，并以另一个量级（1.3 MPa）施加1、3和7天。使用TdT-dUTP末端缺口末端标记（TUNEL）反应对椎间盘的正中矢状切片进行细胞凋亡染色。结果：细胞死亡率的概率单位转换与压应力的对数转换和加载时间的对数转换之和成正比。本研究的结果证明了建立脊柱负荷和椎间盘退变之间定量相关性的可行性。这种相关性可能会在未来耦合到现有的工程模型，预测脊柱负荷响应物理暴露，并导致改善健康和不健康的脊柱负荷的界限的定义，并及时，下背部安全的细化指南。
Study Design. An in vivo study of the toxic consequences of static compressive stress on the intervertebral disc.Objectives. To determine whether disc cell death is correlated with the magnitude and duration of spinal compressive loading.Summary of Background Data. Static compression in vivo has been demonstrated to induce cell death. Cell death, in turn, has been associated with disc degeneration humans. There are currently no tolerance criteria for the intervertebral disc that combine both biomechanical and biologic factors, although both have been implicated in cases of accelerated degeneration.Methods. Mouse tail discs were loaded in vivo with an external compression device. Compressive stress was applied at one of two magnitudes (0.4 acid 0.8 MPa) for 7 days, and at one additional magnitude (1.3 MPa) for 1, 3, and 7 days. Midsagittal sections of the discs were stained for apoptosis using the TdT-dUTP terminal nick-end labeling (TUNEL) reaction. Quantal analysis was used to correlate the extent of cell death to the magnitude and duration of loading.Results: The probit transformation of the percentage of dying cells was proportional to the sum of the logarithmic transformations of the compressive stress and the time of loading.Conclusions. The results of this study demonstrate the feasibility of developing a quantitative correlation between spinal loading and disc degeneration. Such a correlation may be coupled in the future to existing engineering models that predict spinal loading in response to physical exposures and lead to improved definition of the bounds of healthy and unhealthy spinal loading, and uitimately, refined guidelines for low back safety.