Astrocytes express type VIII collagen during the repair process of brain cold injury
Astrocytes express type VIII collagen during the repair process of brain cold injury
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DOI：
10.1016/j.bbrc.2004.03.049
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发表时间：
2004-04-30
影响因子：
3.1
通讯作者：
Ninomiya, Y
中科院分区：
文献类型：
--
作者：
Hirano, S;Yonezawa, T;Ninomiya, Y
我们首次在小鼠脑冷损伤模型修复过程中发现了VIII型胶原基因表达上调。免疫组化染色显示坏死区周围有VIII型胶原表达，并可见反应性星形胶质细胞。培养的星形胶质细胞显示出VIII型胶原基因的高表达。tgf - β 1增强星形胶质细胞α 1（VIII）和α 2（VIII）基因的表达。此外，我们测试了VIII型胶原蛋白的生物活性，并与I、IV、V型胶原蛋白和纤维连接蛋白进行了比较。星形胶质细胞通过需要金属离子的受体粘附在VIII型胶原上。星形胶质细胞在VIII型胶原上的迁移比在其他ECM分子上的迁移更受刺激。这些数据表明，在星形胶质细胞修复过程中，VIII型胶原在胶质瘢痕形成中起重要作用。(C) 2004爱思唯尔公司版权所有。
We recognized for the first time upregulation of type VIII collagen gene expression during the repair process in the mouse brain cold injury model. Immunohistochemical staining showed that type VIII collagen expression was around the necrotic region, where reactive astrocytes are frequently observed. Cultured astrocytes demonstrated a high expression of type VIII collagen genes. TGF-beta1 enhanced the expression of both alpha1(VIII) and alpha2(VIII) genes by astrocytes in culture. Further, we tested selected biological activities of type VIII collagen, compared with those of type I, IV, and V collagens and fibronectin. Astrocytes adhered to type VIII collagen via receptors requiring metal ions. Astrocyte migration on type VIII collagen was more stimulated than that observed on the other ECM molecules. These data indicate that type VIII collagen plays an important role in glial scar formation during the repair process by astrocytes. (C) 2004 Elsevier Inc. All rights reserved.