Ω3-Polyunsaturated fatty acids prevent lipoperoxidation, modulate antioxidant enzymes, and reduce lipid content but do not alter glycogen metabolism in the livers of diabetic rats fed on a high fat thermolyzed diet
Ω3-Polyunsaturated fatty acids prevent lipoperoxidation, modulate antioxidant enzymes, and reduce lipid content but do not alter glycogen metabolism in the livers of diabetic rats fed on a high fat thermolyzed diet
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DOI：
10.1007/s11010-011-1099-4
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发表时间：
2012-02-01
影响因子：
4.3
通讯作者：
Moreira, Jose C.
中科院分区：
文献类型：
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作者：
de Assis, Adriano M.;Rech, Anderson;Moreira, Jose C.
已知Omega 3-多不饱和脂肪酸（Omega 3-PUFA）可作为降血脂剂，但文献不清楚Omega 3-PUFA对肥胖和糖尿病患者氧化应激的影响。在这项研究中，我们的目的是调查的氧化应激，包括抗氧化酶的活性和肝脏脂质和糖原代谢的糖尿病和非糖尿病大鼠喂养高脂饮食的欧米茄3-多不饱和脂肪酸的影响。将大鼠分为六组：（1）对照组（C），（2）对照糖尿病组（D），（3）高脂饮食治疗组（HFTD），其被喂食富含脂肪的饮食并在180 ℃加热60分钟，（4）高脂饮食治疗糖尿病组高脂饮食+ Omega 3多不饱和脂肪酸组（HFTD + Omega 3）;高脂饮食+ Omega 3糖尿病组（D + HFTD + Omega 3）。这项研究最重要的发现是，Omega 3-PUFAs能够降低喂食HFTD的糖尿病大鼠肝脏中的甘油三酯、非酯化脂肪酸、脂质过氧化水平、晚期糖基化终产物、SOD/CAT酶比和CAT免疫含量，并增加SOD 2水平。然而，Omega 3-PUFAs并没有改变肝脏中蛋白质损伤、血糖或糖原代谢的观察水平。这些发现表明，Omega 3-PUFAs可能是对抗糖尿病、胰岛素抵抗和非酒精性脂肪肝等疾病的重要辅助佐剂。
Omega 3-Polyunsaturated fatty acids (Omega 3-PUFAs) are known to act as hypolipidaemics, but the literature is unclear about the effects that Omega 3-PUFAs have on oxidative stress in obese and diabetic patients. In this study, our aim was to investigate the effects of Omega 3-PUFAs on oxidative stress, including antioxidant enzyme activity and hepatic lipid and glycogen metabolism in the livers of diabetic and non-diabetic rats fed on a high fat thermolyzed diet. Rats were divided into six groups: (1) the control group (C), (2) the control diabetic group (D), (3) the high fat thermolyzed diet group (HFTD), which were fed a diet that was enriched in fat that was heated for 60 min at 180A degrees C, (4) the high fat thermolyzed diet diabetic group (D + HFTD), (5) the high fat thermolyzed diet + Omega 3 polyunsaturated fatty acid group (HFTD + Omega 3), and (6) the high fat thermolyzed diet + Omega 3 polyunsaturated fatty acid diabetic group (D + HFTD + Omega 3). The most important finding of this study was that Omega 3-PUFAs are able to reduce triglycerides, non-esterified fatty acid, lipoperoxidation levels, advanced glycation end products, SOD/CAT enzymatic ratio, and CAT immunocontent and increase SOD2 levels in the livers of diabetic rats fed with a HFTD. However, Omega 3-PUFAs did not alter the observed levels of protein damage, blood glucose, or glycogen metabolism in the liver. These findings suggest that Omega 3-PUFAs may represent an important auxiliary adjuvant in combating some diseases like diabetes mellitus, insulin resistance, and non-alcoholic fatty liver disease.