Identification of fusarium head blight resistance related metabolites specific to doubled-haploid lines in barley
Identification of fusarium head blight resistance related metabolites specific to doubled-haploid lines in barley
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DOI：
10.1007/s10658-013-0302-8
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发表时间：
2014-01-01
影响因子：
1.8
通讯作者：
Choo, Thin M.
中科院分区：
文献类型：
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作者：
Chamarthi, Sivakumar K.;Kumar, Kundan;Choo, Thin M.
禾谷镰刀菌产生的镰刀菌赤霉病（FHB）和脱氧雪腐镰刀菌烯醇（DON）毒素在世界范围内降低大麦产量和品质。在小麦和大麦中已经发现了数百个数量性状基因座（QTL），但其功能尚不清楚。应用代谢谱来更好地理解大麦的抗性机制，并鉴定大麦中潜在的FHB抗性生物标志物代谢物。用病原菌或模拟溶液接种4个抗赤霉病大麦（H15-2、H148-3、H203-2和H379-2）和1个感病大麦（H97-2）的双单倍体（DH）品系。病害严重程度量化为疾病进展曲线下面积（AUDPC）显着不同的抗性和敏感基因型之间，但不是在抗性基因型。各品系间DON含量及脱毒产物占总DON的比例均不显著。抗性相关代谢物（RR，抗性比敏感的丰度高）在DH抗性品系之间的数量和倍数变化不同。共选择了144种RR组成型（RRC）和167种RR诱导型（RRI）代谢物，其中分别推定鉴定了39种和37种。这些RR代谢产物主要属于6个化学基团：苯丙素类、羟基肉桂酸酰胺类、黄酮类、脂肪酸类、萜类和生物碱类。特定的RR代谢物确定在每个DH系，可能的机制，在每个和他们的使用作为潜在的生物标志物的阻力进行了讨论。
Fusarium head blight (FHB) and deoxynivalenol (DON) mycotoxin produced by Fusarium graminearum reduce barley yield and quality worldwide. Hundreds of quantitative trait loci (QTLs) have been identified in wheat and barley but their functions are largely unknown. Metabolic profiling was applied to better understand the mechanisms of resistance and to identify potential FHB resistance biomarker metabolites in barley. Four FHB resistant (H15-2, H148-3, H203-2 and H379-2) and one susceptible (H97-2), two-row, purple, doubled-haploid (DH) lines of barley were inoculated with either the pathogen or mock-solution. The disease severity quantified as the area under the disease progress curve (AUDPC) significantly varied between the resistant and susceptible genotypes, but not among the resistant genotypes. Neither the amount of DON nor the detoxified product, proportion of total DON, was significant among lines. The resistance related (RR, higher in abundance in resistant than in susceptible) metabolites varied in numbers and fold changes among the DH resistant lines. A total of 144 RR constitutive (RRC) and 167 RR induced (RRI) metabolites were selected, of which 39 and 37, respectively, were putatively identified. These RR metabolites mainly belonged to six chemical groups: phenylpropanoids, hydroxycinnamic acid amides, flavonoids, fatty acids, terpenoids, and alkaloids. The specific RR metabolites identified in each DH line, the possible mechanisms of resistance in each and their use as potential biomarkers are discussed.