Identification of two blast resistance genes in a rice variety, Digu
Identification of two blast resistance genes in a rice variety, Digu
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DOI：
10.1046/j.1439-0434.2003.00803.x
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发表时间：
2004-02-01
影响因子：
1.5
通讯作者：
Zhu, LH
中科院分区：
文献类型：
--
作者：
Chen, XW;Li, SG;Zhu, LH
稻瘟病是由稻瘟病菌（Magnaporthe grisea）引起的世界性水稻病害之一。地谷是我国地方水稻品种，具有持久的抗瘟性，是水稻抗瘟育种的重要资源之一。grisea）在中国的分布。本研究的目的是评估的身份，抗性基因在Digu和确定染色体定位的分子标记标签。两个感稻瘟病品种丽江新团黑谷（LTH）和江南香糯（JNXN）是一组不同的品种，每个品种都含有一个抗稻瘟病基因Pik（s）、Pia、Pik、Pi-b、Pi-k（p）、Pi-ta（2）、Pi-ta、Pi-z、Pi-i、Pi-k（m）、Pi-z（t）、Pi-t和Pi-11，用中国稻瘟病菌分离物分析了地谷与这些品种杂交的后代群体。结果表明，地谷对中国稻瘟病菌株系ZB 13和ZB 15的抗性分别由两对显性单基因控制。这两个基因不同于已知的稻瘟病抗性基因，因此被命名为Pi-d（t）1和Pi-d（t）2。利用群体分离法和分子标记分析，将Pi-d（t）1定位在第2染色体上，与限制性片段长度多态性（RFLP）标记G1314 A和G45的距离分别为1.2和10.6cM。Pi-d（t）2位于第6号染色体上，与简单重复序列标记RM 527和RM 3的距离分别为3.2和3.4cM。我们还开发了一种新的RGA检测策略，结合不均匀聚合酶链反应（PCR）进一步标记这两个基因，并成功地鉴定了两个RGA标记，SPO 01和SPO 03，它们分别与Pi-d（t）1和Pi-d（t）2在其相应的F-2群体中共分离。这些结果为进一步利用地谷抗稻瘟病基因进行水稻抗病育种和定位克隆提供了必要的信息。
Blast, caused by Magnaporthe grisea is one of most serious diseases of rice worldwide. A Chinese local rice variety, Digu, with durable blast resistance, is one of the important resources for rice breeding for resistance to blast (M. grisea) in China. The objectives of the current study were to assess the identity of the resistance genes in Digu and to determine the chromosomal location by molecular marker tagging. Two susceptible varieties to blast, Lijiangxintuanheigu (LTH) and Jiangnanxiangnuo (JNXN), a number of different varieties, each containing one blast resistance gene, Pik(s), Pia, Pik, Pi-b, Pi-k(p), Pi-ta(2), Pi-ta, Pi-z, Pi-i, Pi-k(m), Pi-z(t), Pi-t and Pi-11, and the progeny populations from the crosses between Digu and each of these varieties were analysed with Chinese blast isolates. We found that the resistance of Digu to each of the two Chinese blast isolates, ZB13 and ZB15, were controlled by two single dominant genes, separately. The two genes are different from the known blast resistance genes and, therefore, designated as Pi-d(t)1 and Pi-d(t)2. By using bulked segregation method and molecular marker analysis in corresponding F-2 populations, Pi-d(t)1 was located on chromosome 2 with a distance of 1.2 and 10.6 cM to restriction fragment length polymorphism (RFLP) markers G1314A and G45, respectively. And Pi-d(t)2 was located on chromosome 6 with a distance of 3.2 and 3.4 cM to simple sequence repeat markers RM527 and RM3, respectively. We also developed a novel strategy of resistance gene analogue (RGA) assay with uneven polymerase chain reaction (PCR) to further tag the two genes and successfully identified two RGA markers, SPO01 and SPO03, which were co-segregated toPi-d(t)1 and Pi-d(t)2, respectively, in their corresponding F-2 populations. These results provide essential information for further utilization of the Digu's blast resistance genes in rice disease resistance breeding and positional cloning of these genes.