High-performance liquid chromatographic analysis of fucoganglioside hydrolysis by alpha-L-fucosidase.
High-performance liquid chromatographic analysis of fucoganglioside hydrolysis by alpha-L-fucosidase.
复制标题
α-L-岩藻糖苷酶水解岩藻神经节苷脂的高效液相色谱分析。
DOI：
10.1016/0003-2697(90)90109-m
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发表时间：
1990
影响因子：
2.9
通讯作者：
Alhadeff,JA
中科院分区：
文献类型：
--
作者：
Johnson,SW;Masserini,M;Alhadeff,JA
建立了一种高效液相色谱法测定α-L-岩藻糖苷酶水解岩藻神经节苷脂的方法。高分辨率方法采用Lichrosorb-NH 2柱，用磷酸钾/乙腈缓冲液等度洗脱10分钟，用195 nm处的紫外监测器检测神经节苷脂产物，并用积分器定量低皮摩尔量的这些神经节苷脂。HPLC方法的有效性已通过使用其证明在不存在活化蛋白和/或去污剂的情况下，纯化的人肝α-L-岩藻糖苷酶对神经节苷脂岩藻糖基-GM 1和岩藻糖基-GD 1的水解来举例说明。
A sensitive HPLC method has been developed for monitoring fucoganglioside hydrolysis by purified α-l-fucosidase. The high-resolution method employs a Lichrosorb-NH2column, a 10-min isocratic elution with potassium phosphate/acetonitrile buffer, detection of ganglioside products with a uv monitor at 195 nm, and quantification of low picomolar amounts of these gangliosides with an integrator. The usefulness of the HPLC method has been exemplified by using it to demonstrate the hydrolysis of gangliosides fucosyl-GM1and fucosyl-GD1bby purified human liver α-l-fucosidase in the absence of activator proteins and/or detergents.