Constructed wetlands as hotspots of antibiotic resistance genes and pathogens: Evidence from metagenomic analysis in Chinese rural areas.
Constructed wetlands as hotspots of antibiotic resistance genes and pathogens: Evidence from metagenomic analysis in Chinese rural areas.
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DOI：
10.1016/j.jhazmat.2023.130778
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发表时间：
2023-01
影响因子：
13.6
通讯作者：
Nan Zhang;D. Lu;Huafeng Sheng;Jingjing Xia;Peiying Kan;Z. Yao;Huaihai Chen;Gang Li;David Z Zhu;Hongzhi Liu
中科院分区：
文献类型：
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作者：
Nan Zhang;D. Lu;Huafeng Sheng;Jingjing Xia;Peiying Kan;Z. Yao;Huaihai Chen;Gang Li;David Z Zhu;Hongzhi Liu
在中国农村，开发了许多人工湿地（CW）来可持续地处理农村废水。然而，由于这些农村化武场的信息匮乏，很难分析这些系统内的生物污染物，例如抗生素抗性基因（ARG）和病原体。本研究基于两个中试规模、观察一年的CW收集的数据，首次利用宏基因组测序方法和SourceTracker分析在不同水力负荷率下探索了ARG和病原体的积累。废水中 ARG 的香农指数超过了进水中的水平。 DESeq2 分析显示，与进水相比，出水中高达 21.49% 的总病原体种类的相对丰度有所增加。通过结合基质和根际的贡献，CW 成为比进水更能影响 ARG 和病原体污染的因素。网络分析揭示了一个关键但潜在的事实，即抗生素耐药性病原体的发展很可能是由 ARG 和病原体的共存引发的。总的来说，从生物风险的角度来看，我们的研究表明，仅靠化学水可能不是改善中国农村废水处理的理想解决方案。
In rural China, many constructed wetlands (CWs) have been developed to treat rural wastewater sustainably. However, due to the scarce information on those rural CWs, it is difficult to analyze the biological contaminants within those systems, such as antibiotic resistance genes (ARGs) and pathogens. Based on the data collected from two pilot-scale, one-year-observed CWs, for the first time, this study explored the accumulation of ARGs and pathogens using the metagenomic sequencing approach and SourceTracker analysis under different hydraulic loading rates. The Shannon index of ARGs in the effluent surpassed the level found in the influent. The DESeq2 analysis showed that up to 21.49% of the total pathogen species had increased relative abundance in the effluent compared with the influent. By combining the contribution of substrate and rhizosphere, the CW became a more influencing factor for ARGs and pathogens contamination than the influent. The network analysis revealed a critical but latent fact that the development of antibiotic-resistant pathogens is highly likely to be triggered by the co-occurrence of ARGs and pathogens. Collectively, from the aspect of biological risk, our study showed that CWs alone might not be an ideal solution for improving wastewater treatment in rural China.