NETs formed by human neutrophils inhibit growth of the pathogenic mold Aspergillus fumigatus
NETs formed by human neutrophils inhibit growth of the pathogenic mold Aspergillus fumigatus
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DOI：
10.1016/j.micinf.2010.06.009
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发表时间：
2010-11-01
影响因子：
5.8
通讯作者：
Ebel, Frank
中科院分区：
文献类型：
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作者：
McCormick, Allison;Heesemann, Leonie;Ebel, Frank
中性粒细胞胞外陷阱（NETs）代表了控制和消除微生物感染的独特机制。我们的研究结果表明，人类致病性霉菌烟曲霉的分生孢子和芽管能够触发NETs的形成。这种宿主病原体相互作用基本上不需要活的真菌细胞。中性粒细胞吞噬分生孢子，从而抑制它们的萌发，一个独立于NETosis的过程在本研究中使用的实验装置中，中性粒细胞不杀死芽管，但减少其极性生长，并且这种抑制依赖于NETs，因为它可以通过添加DNase 1来克服。诱导的NET和添加Zn 2+消除NET介导的生长抑制总之，我们的数据提供了证据表明NET不足以杀死烟曲霉，但可能是限制感染的有价值的工具（C）2010 Institut Pasteur Published by Elsevier Masson SAS版权所有
Neutrophil extracellular traps (NETs) represent a distinct mechanism to control and eliminate microbial infections Our results show that conidia and germ tubes of the human pathogenic mold Aspergillus fumigatus are able to trigger the formation of NETs Viable fungal cells are not essentially required for this host pathogen interaction Neutrophils engulf conidia and thereby inhibit their germination, a process that is independent of NETosis In the experimental set-up used in this study neutrophils do not kill germ tubes but reduce their polar growth and this inhibition depends on NETs as it can be overcome by the addition of DNase 1 The Zn2+ chelator calprotectin is associated with the Aspergdlus-induced NETs and addition of Zn2+ abrogates the NET-mediated growth inhibition In summary, our data provide evidence that NETs are not sufficient to kill A fumigatus but might be a valuable tool to confine infection (C) 2010 Institut Pasteur Published by Elsevier Masson SAS All rights reserved