Susceptibility to three pyrethroids and detection of knockdown resistance mutation in Ghanaian Anopheles gambiae sensu stricto
Susceptibility to three pyrethroids and detection of knockdown resistance mutation in Ghanaian Anopheles gambiae sensu stricto
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DOI：
10.3376/1081-1710-33.2.255
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发表时间：
2008-12-01
影响因子：
1.7
通讯作者：
Hemingway, Janet
中科院分区：
文献类型：
--
作者：
Adasi, Kofi;Hemingway, Janet
虽然杀虫剂在疟疾控制方案中发挥着至关重要的作用，但这一战略受到了病媒对常用公共卫生杀虫剂产生抗药性的威胁。由于其已知的功效和对人类和非目标生物的较低毒性，拟除虫菊酯已被用于许多病媒控制项目。在西非，几个国家报告了主要疟疾病媒冈比亚按蚊对拟除虫菊酯的抗药性。因此，必须调查加纳这一非常重要的疟疾病媒的易感性，并确定其抗药性机制。从农村和城市地点收集广义冈比亚按蚊幼虫，并饲养至成虫。通过形态特征、PCR和HhaI酶切鉴定种属。选择2至3天大的雌蚊，并使其接触世界卫生组织（世卫组织）诊断剂量的三种拟除虫菊酯。一种敏感的实验室菌株。严格意义上的冈比亚被用作参照。对来自生物测定的存活蚊子和死亡蚊子筛选敲低抗性突变。一个.严格意义上的冈比亚种是这些地区存在的复合体的唯一姊妹种，分子S型占优势（>95%）。观察到对拟除虫菊酯的抗性高达8.5倍，具有非常高的kdr频率。由于分子技术的使用相对容易，疟疾病媒中表面上的杀虫剂抗性基因得以快速检测。然而，更重要的是，在现场进行常规杀虫剂测试，以补充这些分子工具，特别是如果杀虫剂被指定用于公共卫生用途。Journal of Vector Ecology 33（2）：255-262. 2008.
Although insecticides play a crucial role in malaria control programs, this strategy is threatened by the development of resistance in the vectors to commonly used public health insecticides. Due to their known efficacy and lower toxicity to humans and non-target organisms, pyrethroids have been used in many vector control projects. In West Africa, pyrethroid resistance in the major malaria vector, Anopheles gambiae, had been reported in several countries. It was, therefore, imperative to investigate the susceptibility of this very important malaria vector in Ghana and characterize the resistance mechanism. Larvae of Anopheles gambiae sensu lato were collected from rural and urban sites and reared to adults. Species identification was by morphological characteristics, PCR, and HhaI restriction digest. Female mosquitoes that were two to three days old were selected and exposed to World Health Organization (WHO) diagnostic doses of three pyrethroids. A susceptible laboratory strain of An. gambiae sensu stricto was used as a reference. Both survivors and dead mosquitoes from bioassays were screened for the knockdown resistance mutation. An. gambiae sensu stricto was the only sibling species of the complex present in these localities with the molecular S form being predominant (>95%). Resistance to pyrethroids up to 8.5 fold was observed, with very high kdr frequency. The relative ease in using molecular techniques has resulted in rapid detection of ostensible insecticide resistance genes in malaria vectors. However, it is even more important to complement these molecular tools with routine insecticide testing in the field, especially if the insecticides are earmarked for public health use. Journal of Vector Ecology 33 (2): 255-262. 2008.