Galacto-oligosaccharides may directly enhance intestinal barrier function through the modulation of goblet cells
Galacto-oligosaccharides may directly enhance intestinal barrier function through the modulation of goblet cells
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DOI：
10.1002/mnfr.201400639
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发表时间：
2015-03-01
影响因子：
5.2
通讯作者：
Gaskins, H. Rex
中科院分区：
文献类型：
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作者：
Bhatia, Shikha;Prabhu, P. Nagendra;Gaskins, H. Rex
范围：在这里，我们测试了益生元低聚半乳糖（GOS）可以通过直接调节杯状细胞功能来增强粘膜屏障功能的假设。方法和结果：使用表现出肠杯状细胞样表型的人腺癌来源的 LS174T 细胞来检查 GOS 对杯状细胞功能的非益生元影响。用GOS处理LS174T细胞，杯状细胞分泌产物基因粘蛋白2（MUC2）、三叶因子3（TFF3）、抵抗素样分子β（RETNLB）和高尔基体磺基转移酶基因、碳水化合物（N-乙酰葡糖胺-6-O）磺基转移酶5（CHST5）和半乳糖-3-O-磺基转移酶2的表达(GAL3ST2)，通过实时定量RT-PCR测定。此外，通过蛋白质印迹分析证实了 CHST5、TFF3 和 RETNLB 的丰度。 GOS处理72小时后，MUC2的表达显着上调2-4倍，CHST5和RETNLB显着上调5-7倍，TFF3表达显着上调2-4倍。蛋白质印迹分析表明 RETNLB、TFF3 和 CHST5 丰度增加。添加 Th2 细胞因子 IL-13 和 GOS 导致 RETNLB 和 CHST5 的协同诱导。 IL-8分泌不受GOS治疗的影响，表明GOS的作用不是通过炎症途径介导的。结论：总的来说，数据表明GOS可能通过直接刺激肠杯状细胞来增强粘膜屏障功能。
Scope: Here we have tested the hypothesis that prebiotic galacto-oligosaccharides (GOS) may enhance mucosal barrier function through direct modulation of goblet cell function.Methods and results: Human adenocarcinoma-derived LS174T cells, which exhibit an intestinal goblet cell-like phenotype, were used to examine the non-prebiotic effects of GOS on goblet cell functions. LS174T cells were treated with GOS, and the expression of goblet cell secretory product genes mucin 2 (MUC2), trefoil factor 3 (TFF3), resistin-like molecule beta (RETNLB) and the Golgi-sulfotransferase genes, carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 5 (CHST5) and galactose-3-O-sulfotransferase 2 (GAL3ST2), was determined by real-time quantitative RT-PCR. In addition, the abundance of CHST5, TFF3 and RETNLB was confirmed by Western blot analysis. Following treatment with GOS for 72 h, the expression of MUC2 was significantly upregulated 2-4-fold, CHST5 and RETNLB, 5-7-fold, and TFF3 2-4-fold. Western blot analysis demonstrated increased abundance of RETNLB, TFF3 and CHST5. Addition of the Th2 cytokine IL-13 along with GOS resulted in synergistic induction of RETNLB and CHST5. IL-8 secretion was not affected by GOS treatment, suggesting that the effects of GOS are not mediated through an inflammatory pathway.Conclusion: Collectively, the data indicate that GOS may enhance mucosal barrier function through direct stimulation of intestinal goblet cells.