Diverse contribution of amniogenic somatopleural cells to cardiovascular development: With special reference to thyroid vasculature
Diverse contribution of amniogenic somatopleural cells to cardiovascular development: With special reference to thyroid vasculature
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羊膜胸膜细胞对心血管发育的多种贡献：特别涉及甲状腺脉管系统
DOI：
10.1002/dvdy.532
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发表时间：
2022
影响因子：
2.5
通讯作者：
Kurihara Hiroki
中科院分区：
文献类型：
--
作者：
Haneda Yuka;Miyagawa‐Tomita Sachiko;Uchijima Yasunobu;Iwase Akiyasu;Asai Rieko;Kohro Takahide;Wada Youichiro;Kurihara Hiroki
研究背景体侧板是羊膜的原基，羊膜是包围胚胎的胚外膜。最近，我们报道了促分化的体侧胸膜细胞（ASCs）不仅形成羊膜，而且可以迁移到胚胎中并分化为心肌细胞和血管内皮细胞。然而，详细的分化过程和这些胚胎内ASCs（以下简称iASCs）的最终分布仍然在很大程度上unknown.ResultsBy鹌鹑小鸡嵌合体分析，我们在这里表明，iASCs分化成各种细胞类型，包括心肌细胞，平滑肌细胞，心脏间质细胞和血管内皮细胞。在咽部区域，它们选择性地分布到甲状腺中并分化成血管内皮细胞以形成甲状腺内脉管系统。外植体培养实验表明，iASCs的内皮分化依次需要成纤维细胞生长因子（FGF）和血管内皮生长因子（VEGF）信号。单细胞转录组分析进一步揭示了异质性和ASCs内血管母细胞样细胞群的存在，从FGF到VEGF受体基因expression.ConclusionThe本研究表明ASCs的新作用，特别是在心脏和甲状腺发育。这将为从胚胎学和进化的角度了解蝾螈的心血管发育提供新的线索。
BackgroundThe somatopleure serves as the primordium of the amnion, an extraembryonic membrane surrounding the embryo. Recently, we have reported that amniogenic somatopleural cells (ASCs) not only form the amnion but also migrate into the embryo and differentiate into cardiomyocytes and vascular endothelial cells. However, detailed differentiation processes and final distributions of these intra‐embryonic ASCs (hereafter referred to as iASCs) remain largely unknown.ResultsBy quail‐chick chimera analysis, we here show that iASCs differentiate into various cell types including cardiomyocytes, smooth muscle cells, cardiac interstitial cells, and vascular endothelial cells. In the pharyngeal region, they distribute selectively into the thyroid gland and differentiate into vascular endothelial cells to form intra‐thyroid vasculature. Explant culture experiments indicated sequential requirement of fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF) signaling for endothelial differentiation of iASCs. Single‐cell transcriptome analysis further revealed heterogeneity and the presence of hemangioblast‐like cell population within ASCs, with a switch from FGF to VEGF receptor gene expression.ConclusionThe present study demonstrates novel roles of ASCss especially in heart and thyroid development. It will provide a novel clue for understanding the cardiovascular development of amniotes from embryological and evolutionary perspectives.