Characterization of Ferroplasma isolates and Ferroplasma acidarmanus sp nov., extreme acidophiles from acid mine drainage and industrial bioleaching environments
Characterization of Ferroplasma isolates and Ferroplasma acidarmanus sp nov., extreme acidophiles from acid mine drainage and industrial bioleaching environments
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DOI：
10.1128/aem.70.4.2079-2088.2004
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发表时间：
2004-04-01
影响因子：
4.4
通讯作者：
Bond, PL
中科院分区：
文献类型：
--
作者：
Dopson, M;Baker-Austin, C;Bond, PL
通过16S rRNA基因测序，最近分离的三株极端嗜酸古菌菌株与嗜酸铁原体Y-T具有相似的系统发育特征。所有四种铁原体分离株都能在酵母提取物或一系列糖上进行化学有机营养生长，在亚铁和酵母提取物或糖上进行化学混合营养生长，并且分离株“酸性铁原体”Fer1(T)需要更高水平的有机碳。所有4株菌株均为兼性厌氧菌，在酵母提取物上进行化学有机营养生长以还原铁。4株菌株的最适温度为35 ~ 42℃，最适pH为1.0 ~ 1.7，“F. acidarmanus”Fer1(T)能在pH为0的条件下生长。“F. acidarmanus”Fer1(T)的最佳酵母浸出物浓度高于其他3个菌株。表型结果表明，分离菌株“F. acidarmanus”Fer1(T)与其他3株属不同种，16S rRNA序列数据、DNA-DNA相似性值和二维聚丙烯酰胺凝胶电泳蛋白谱明确显示菌株DR1、MT17和Y-T属同一种。“F. acidarmanus”Fer1(T)类群分离，提出新种“F. acidarmanus”Fer1(T) sp. nov。
Three recently isolated extremely acidophilic archaeal strains have been shown to be phylogenetically similar to Ferroplasma acidiphilum Y-T by 16S rRNA gene sequencing. All four Ferroplasma isolates were capable of growing chemoorganotrophically on yeast extract or a range of sugars and chemomixotrophically on ferrous iron and yeast extract or sugars, and isolate "Ferroplasma acidarmanus" Fer1(T) required much higher levels of organic carbon. All four isolates were facultative anaerobes, coupling chemoorganotrophic growth on yeast extract to the reduction of ferric iron. The temperature optima for the four isolates were between 35 and 42degreesC and the pH optima were 1.0 to 1.7, and "F. acidarmanus" Fer1(T) was capable of growing at pH 0. The optimum yeast extract concentration for "F. acidarmanus" Fer1(T) was higher than that for the other three isolates. Phenotypic results suggested that isolate "F. acidarmanus" Fer1(T) is of a different species than the other three strains, and 16S rRNA sequence data, DNA-DNA similarity values, and two-dimensional polyacrylamide gel electrophoresis protein profiles clearly showed that strains DR1, MT17, and Y-T group as a single species. "F. acidarmanus" Fer1(T) groups separately, and we propose the new species "F. acidarmanus" Fer1(T) sp. nov.