STRUCTURE OF THE DROSOPHILA DNA TOPOISOMERASE-II GENE - NUCLEOTIDE-SEQUENCE AND HOMOLOGY AMONG TOPOISOMERASES-II
STRUCTURE OF THE DROSOPHILA DNA TOPOISOMERASE-II GENE - NUCLEOTIDE-SEQUENCE AND HOMOLOGY AMONG TOPOISOMERASES-II
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DOI：
10.1016/0022-2836(89)90361-6
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发表时间：
1989-01-05
影响因子：
5.6
通讯作者：
HSIEH, TS
中科院分区：
文献类型：
--
作者：
WYCKOFF, E;NATALIE, D;HSIEH, TS
我们已经确定了果蝇DNA拓扑异构酶II基因的核苷酸序列。引物延伸和S1核酸酶保护实验的数据与基因组和cDNA序列的比较相结合，以确定成熟信使RNA的结构。该信息具有4341个核苷酸的大开放阅读框。预测的蛋白质长度为1447个氨基酸，分子量为164，424。拓扑异构酶II可分为三个结构域：（1）与细菌II型拓扑异构酶DNA促旋酶的B（ATP酶）亚基具有同源性的N-末端区域;（2）与DNA促旋酶的A（断裂和重新连接）亚基具有同源性的中心区域;（3）以带正电荷和带负电荷的氨基酸的交替延伸为特征的C-末端区域。果蝇的DNA拓扑异构酶II与来自不同来源的DNA拓扑异构酶II具有显著的序列同源性，包括细菌、噬菌体T4和酵母。分析了这些序列中同源延伸的位置和分布。
We have determined the nucleotide sequence of the Drosophila DNA topoisomerase II gene. Data from primer extension and S1 nuclease protection experiments were combined with comparisons of genomic and cDNA sequences to determine the structure of the mature messenger RNA. This message has a large open reading frame of 4341 nucleotides. The length of the predicted protein is 1447 amino acids with a molecular weight of 164,424. Topoisomerase II can be divided into three domains: (1) an N-terminal region with homology to the B (ATPase) subunit of the bacterial type II topoisomerase, DNA gyrase; (2) a central region with homology to the A (breaking and rejoining) subunit of DNA gyrase; (3) a C-terminal region characterized by alternating stretches of positively and negatively charged amino acids. DNA topoisomerase II from the fruit fly shares significant sequence homology with those from divergent sources, including bacteria, bacteriophage T4 and yeasts. The location and distribution of homologous stretches in these sequences are analyzed.