Regulation of one type of Ca2+ current in smooth muscle cells by diacylglycerol and acetylcholine.
Regulation of one type of Ca2+ current in smooth muscle cells by diacylglycerol and acetylcholine.
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二酰甘油和乙酰胆碱对平滑肌细胞中一种 Ca2 电流的调节。
DOI：
10.1096/fasebj.2.9.2453389
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Singer,JJ
中科院分区：
文献类型：
--
作者：
Vivaudou,MB;Clapp,LH;WalshJr,JV;Singer,JJ
新鲜分离的蟾蜍胃平滑肌细胞的电生理记录显示有两种类型的钙电流。一种激活阈值低，失活速度快；另一种激活阈值高，失活较慢。乙酰胆碱(ACh)增加高阈值电流，但不增加低阈值电流。蛋白激酶C(PKC)激活剂n-1，2-二辛酰甘油的合成类似物可模拟ACh对钙电流的影响。然而，另一种结构密切相关但不激活PKC的二酰基甘油类似物1，2-二辛酰基-3-硫代甘油不能增加钙电流。L的情况也是如此，它是一种类似物，即使在高浓度下也只能最低限度地激活蛋白激酶C。这些结果表明，二酰甘油可能是ACh对一种电压激活的钙通道的第二信使，可能通过激活PKC-Vivaudou，M.B.；Clapp，L.H.；Walsh，J.V.，Jr.；Singer，J.用二酰甘油和乙酰胆碱调节一种类型的钙电流.FASE B J.2：2497-2504；1988.
Electrophysiological recordings from freshly dissociated smooth muscle cells from the stomach of the toadBufo marinusrevealed two types of Ca2+currents. One has a low threshold of activation and inactivates rapidly; the other has a high threshold of activation and inactivates more slowly. Acetylcholine (ACh) increased the high‐threshold current but not the low‐threshold current. The synthetic diacylglycerol analogsn‐1,2‐dioctanoylglycerol, an activator of protein kinase C (PKC), mimicked these effects of ACh on Ca2+currents. However, another diacylglycerol analog, 1,2‐dioctanoyl‐3‐thioglycerol, which has a closely related structure but does not activate PKC, failed to increase the Ca2+current. The same was true of l,2‐dioctanoyl‐3‐chloropropanediol, an analog that even at high concentrations only minimally activates PKC. These results suggest that diacylglycerol may be the second messenger mediating the effects of ACh on one type of voltage‐activated Ca2+channel, possibly by activating PKC.— Vivaudou, M. B.; Clapp, L. H.; Walsh, J. V., Jr.; Singer, J. J. Regulation of one type of Ca2+current in smooth muscle cells by diacylglycerol and acetylcholine.FASEB J.2: 2497‐2504; 1988.