Solid-State NMR Investigations of Membrane-Associated Antimicrobial Peptides
Solid-State NMR Investigations of Membrane-Associated Antimicrobial Peptides
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DOI：
10.1007/978-1-60761-594-1_14
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Bechinger, Burkhard
中科院分区：
文献类型：
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作者：
Aisenbrey, Christopher;Bertani, Philippe;Bechinger, Burkhard
固态核磁共振和其他生物物理研究揭示了许多关于抗菌肽与膜相互作用的机理细节。这些研究已经形成了我们对这些肽如何杀死细菌，真菌或肿瘤细胞以及它们如何渗透模型膜的看法。因此，WC更好地了解这些肽的生物活性，我们现在能够设计新的和更好的序列。在这里，WC介绍了一些允许这些固态NMR研究的工具，包括如何将肽重组成定向或非定向膜的详细协议，以及H-2和质子去耦P-31或N-15固态NMR测量的简单设置程序。介绍了静态和魔角旋转实验。在适当的情况下，一些测量的特殊要求或限制进行了讨论。固态核磁共振谱的脂质和肽已被记录，并通过合奏的测量这些复杂的肽脂质超分子系统的详细图片终于出现。
Solid-state NMR and other biophysical investigations have revealed many mechanistic details about the interactions of antimicrobial peptides with membranes. These studies have shaped our view on how these peptides cause the killing of bacteria, fungi, or tumour cells and how they permeabilize model membranes. As a result, WC better understand the biological activities of these peptides and we are now able to design new and better sequences. Here WC present some of the tools that have allowed these solid-state NMR investigations, including detailed protocols on how to reconstitute the peptides into oriented Or non-oriented membranes as well as simple set-up procedures for H-2 as well as proton-decoupled P-31 or N-15 solid-state NMR measurements. Static and magic angle spinning experiments are described. Where adequate, the special requirements for or limitations of some of the measurements are discussed. Solid-state NMR spectra of both lipids and peptides have been recorded, and through the ensemble of measurements a detailed picture of these complex peptide lipid supramolecular systems has finally emerged.