Single-nucleotide polymorphisms in FLT3, NLRP5, and TGIF1 are associated with litter size in Small-tailed Han sheep.
Single-nucleotide polymorphisms in FLT3, NLRP5, and TGIF1 are associated with litter size in Small-tailed Han sheep.
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FLT3、NLRP5和TGIF1的单核苷酸多态性与小尾寒羊产羔数相关
DOI：
10.5194/aab-64-475-2021
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发表时间：
2021
影响因子：
1.8
通讯作者：
Chu M
中科院分区：
文献类型：
--
作者：
Chen S;Tao L;He X;Di R;Wang X;Chu M
先前的研究表明，FLT3、NLRP5和TGIF1在绵羊繁殖力中起着关键作用。然而，对这些基因多态性与窝产仔数（LS）的关系知之甚少。所有7个snp的估计等位基因频率的可靠性至少为0.9545。鉴于TGIF1 g。37866222C > T多态性与LS在小尾寒羊中存在差异（p<0.05），繁殖力差异可能是由于氨基酸从脯氨酸（Pro）转变为丝氨酸（Ser），从而影响了二级、三级蛋白结构，同时TGIF1功能发生改变。FLT3 rs421947730位点对LS有显著影响（p<0.05），说明FLT3位点与KILTG协同作用可能促进卵巢卵泡成熟和排卵。NLRP5 rs426897754与第二胎和第三胎的LS相关（p<0.05）。我们推测，NLRP5的同义变体可能与BMP15、FSHR、BMPR1B、AMH和GDF9一起参与卵泡发生，导致小尾寒羊的繁殖力不同。我们的研究为绵羊育种提供了有价值的遗传标记。
Abstract Previous studies have indicated that FLT3, NLRP5, and TGIF1 play a pivotal role in sheep fecundity. Nevertheless, little is known about the association of the polymorphisms of these genes with litter size (LS). In this study, the selected single-nucleotide polymorphisms (SNPs) were genotyped using a Sequenom MassARRAY® platform, and the distribution of different genotypes of the SNPs in the seven sheep breeds (Small-tailed Han, Hu, Cele Black, Suffolk, Tan, Prairie Tibetan, and Sunite sheep) were analyzed. The reliability of the estimated allele frequency for all seven SNPs was at least 0.9545. Given the association of the TGIF1 g.37866222C > T polymorphism with LS in Small-tailed Han sheep (p<0.05), fecundity differences might be caused by the change in amino acid from proline (Pro) to serine (Ser), which has an impact on secondary, tertiary protein structures with concomitant TGIF1 functionality changes. The FLT3 rs421947730 locus has a great effect on the LS (p<0.05), indicating that the locus of FLT3 in synergy with KILTG is likely to facilitate ovarian follicle maturation and ovulation. Moreover, NLRP5 rs426897754 is associated with the LS of the second and third parities (p<0.05). We speculate that a synonymous variant of NLRP5 may be involved in folliculogenesis accompanied by BMP15, FSHR, BMPR1B, AMH, and GDF9, resulting in the different fecundity of Small-tailed Han sheep. Our studies provide valuable genetic markers for sheep breeding.