Introns play an essential role in splicing-dependent formation of the exon junction complex
Introns play an essential role in splicing-dependent formation of the exon junction complex
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DOI：
10.1101/gad.1557907
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发表时间：
2007-08-15
影响因子：
10.5
通讯作者：
Hirose, Tetsuro
中科院分区：
文献类型：
--
作者：
Ideue, Takashi;Sasaki, Yasnory T. F.;Hirose, Tetsuro
前 mRNA 剪接将外显子连接复合物 (EJC) 特异性沉积到剪接的 mRNA 上，这对于下游事件很重要。在这里，我们表明 EJC 成分主要通过内含子结合蛋白 IBP160 与内含子结合而被招募到剪接体。 EJC 组件的这种初始关联发生在外显子上不存在最终 EJC 结合位点的情况下。 IBP160 的 RNA 干扰 (RNAi) 敲低阻止了 EJC 在无义介导的衰变后与细胞质 RNA 的关联。我们认为内含子在 EJC 形成的早期步骤中起着至关重要的作用，并且对于随后功能性 EJC 的形成是不可或缺的。
Pre-mRNA splicing specifically deposits the exon junction complex (EJC) onto spliced mRNA, which is important for downstream events. Here, we show that EJC components are primarily recruited to the spliceosome by association with the intron via the intron-binding protein, IBP160. This initial association of EJC components occurs in the absence of the final EJC-binding site on the exon. RNA interference (RNAi) knockdown of IBP160 arrested EJC association with cytoplasmic RNAs following nonsense-mediated decay. We propose that the intron has a crucial role in the early steps of EJC formation and is indispensable for the subsequent formation of a functional EJC.