Negative Control of rpoS Expression by Phosphoenolpyruvate:Carbohydrate Phosphotransferase System inEscherichia coli
Negative Control of rpoS Expression by Phosphoenolpyruvate:Carbohydrate Phosphotransferase System inEscherichia coli
复制标题
磷酸烯醇丙酮酸：大肠杆菌中碳水化合物磷酸转移酶系统对 rpoS 表达的负控制
作者：
C. Ueguchi;Naoko Misonou;T. Mizuno
摘要rpoS基因编码的RNA聚合酶的α S（或α 38）亚基是一个重要的调节因子，在应激条件下（如营养饥饿），它对一组基因的转录调控起着关键作用。rpoS的表达在转录、翻译和产物稳定性水平上以复杂的方式受到调节。虽然已经鉴定了许多参与rpoS表达调控的因子，但其潜在的分子机制尚未完全了解。在这项研究中，我们确定了Crr（或EIIAGlc）蛋白作为一种新的因素，不仅在转录控制，而且在rpoS表达的翻译控制中发挥重要作用。Crr是葡萄糖摄取的重要组成部分，通过磷酸烯醇丙酮酸：碳水化合物磷酸转移酶系统。一系列的遗传分析结果表明，Crr负调控srpoS的翻译和转录。观察到的转录控制Crr似乎是由环AMP介导的。然而，发现Crr负控制rpos翻译，而直接。这些结果表明rpoS表达的控制和碳代谢之间可能存在联系。
ABSTRACT The ςS (or ς38) subunit of RNA polymerase, encoded by the rpoS gene, is a crucial regulator in the transcriptional control of a set of genes under stressful conditions, such as nutrient starvation. The expression ofrpoS is regulated in a complex manner at the levels of transcription, translation, and stability of the product. Although a number of factors involved in the regulation of rpoSexpression have been identified, the underlying molecular mechanisms are not fully understood. In this study, we identified the Crr (or EIIAGlc) protein as a novel factor that plays an important role not only in the transcriptional control but also in the translational control of rpoS expression. Crr is an important component in glucose uptake through the well-characterized phosphoenolpyruvate:carbohydrate phosphotransferase system. The results of a series of genetic analyses revealed that Crr negatively controlsrpoS translation and transcription. The observed transcriptional control by Crr appears to be mediated by cyclic AMP. However, it was found that Crr negatively controls rpoStranslation rather directly. These results suggest a possible linkage between the control of rpoS expression and carbon metabolism.
通过大肠杆菌乳糖通透酶的半胱氨酸扫描诱变推导结合磷酸烯醇丙酮酸：糖磷酸转移酶系统的 IIAGlc 的蛋白质上的共有结合序列。
DOI：
10.1073/pnas.96.7.3525
发表时间：
1999
影响因子：
11.1
作者：
Sondej,M;Sun,J;Seok,YJ;Kaback,HR;Peterkofsky,A
通讯作者：
Peterkofsky,A
响应调节器 SprE 控制 RpoS 的稳定性。
DOI：
10.1073/pnas.93.6.2488
发表时间：
1996
影响因子：
11.1
作者：
Pratt,LA;Silhavy,TJ
通讯作者：
Silhavy,TJ
转座子的使用，重点是 Tn10。
影响因子：
--
作者：
Kleckner,N;Bender,J;Gottesman,S
通讯作者：
Gottesman,S