Lipid Mediator Metabolomics Via LC-MS/MS Profiling and Analysis
Lipid Mediator Metabolomics Via LC-MS/MS Profiling and Analysis
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DOI：
10.1007/978-1-4939-7592-1_4
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发表时间：
2018-01-01
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影响因子：
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通讯作者：
Serhan, Charles N.
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文献类型：
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作者：
Dalli, Jesmond;Colas, Romain A.;Serhan, Charles N.
固相萃取与液相色谱串联质谱联用为鉴定和定量专门的促消退介质（脂氧素、消退素、保护素和maresins）、其通路标记物和经典类花生酸提供了一种稳健且灵敏的方法。在这里，我们提供了一个详细的描述，这些介质从生物系统中提取的方法，仪器的设置，样品处理，然后为他们的识别和定量所遵循的程序。
Solid-phase extraction coupled with liquid chromatography tandem mass spectrometry provides a robust and sensitive approach for the identification and quantitation of specialized pro-resolving mediators (lipoxins, resolvins, protectins, and maresins), their pathway markers and the classic eicosanoids. Here, we provide a detailed description of the methodologies employed for the extraction of these mediators from biological systems, setup of the instrumentation, sample processing, and then the procedures followed for their identification and quantitation.