Electrochemical detection of protein using a double aptamer sandwich
Electrochemical detection of protein using a double aptamer sandwich
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DOI：
10.1081/al-200035778
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发表时间：
2004-11-01
影响因子：
2
通讯作者：
Sode, K
中科院分区：
文献类型：
--
作者：
Ikebukuro, K;Kiyohara, C;Sode, K
我们开发了一种用于蛋白质检测的电化学传感器系统，使用两种不同的抗凝血酶适体来识别目标蛋白质的不同部分。在这种情况下，使用夹心形式检测凝血酶，该夹心形式由葡萄糖脱氢酶（GDH）标记的抗凝血酶适体和固定在金电极上以将凝血酶捕获到其上的另一个适体组成。测定添加葡萄糖时GDH产生的电流，选择性地检测1μM的凝血酶。
We have developed an electrochemical sensor system for protein detection using two different antithrombin aptamers that recognize different parts of the target protein. In this case, thrombin was detected using a sandwich format comprised of a glucose dehydrogenase (GDH)-labeled antithrombin aptamer and another aptamer that was immobilized onto the gold electrode for capturing the thrombin onto it. The electric current generated by the GDH when glucose was added was measured, and 1 muM of thrombin was detected selectively.