First evidence of Wolbachia infection in populations of grasshopper Podisma sapporensis (Orthoptera: Acrididae)
First evidence of Wolbachia infection in populations of grasshopper Podisma sapporensis (Orthoptera: Acrididae)
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DOI：
10.1111/ens.12187
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发表时间：
2016-07-01
影响因子：
0.9
通讯作者：
Tatsuta, Haruki
中科院分区：
文献类型：
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作者：
Bugrov, Alexander G.;Ilinsky, Yury Yu;Tatsuta, Haruki
短翅型蝗虫Podisma Sapporensis(直翅目：蝗科)分布于库页岛、库纳什岛和北海道群岛。该物种的核型由两个具有不同性染色体系统的主染色体小种组成，XO/XX和XY/XX。染色体小种和亚种的分子系统地理分析证实了小种和亚种间的遗传分化。本文首次报道了由WSP基因座的三个变异体组成的沃尔巴克氏菌，而gatB基因座是单态的。此外，对南美白对虾的细胞组织进行了电子显微镜观察，证实了聚合酶链式反应推测的沃尔巴克氏菌的感染，并揭示了该细菌在南美白对虾胚胎头部、胸部和腹部的分布。我们的发现可能为沃尔巴克氏菌作为一种可能的病原体提供新的线索，该病原体可能是由实验中的萨普兰人染色体小种或亚种之间的杂交引起的杂交功能障碍的原因。
The brachypterous grasshopper Podisma sapporensis (Orthoptera: Acrididae) is distributed throughout the Sakhalin, Kunashir and Hokkaido Islands. Karyotypes of this species consist of two major chromosomal races with different sex chromosome systems, XO/XX and XY/XX. Molecular phylogeographic analysis of the chromosome races and subraces confirms the genetic divergence of the races and subraces in P.sapporensis. Here we first report that P.sapporensis is infected with Wolbachia consisting of three variants on wsp locus, while gatB locus was monomorphic. Furthermore, observation of cell tissue of P.sapporensis using electron microscopy confirmed the infection of Wolbachia that was inferred from polymerase chain reaction and revealed the distribution of the bacteria in the head, thorax and abdomen of P.sapporensis embryos. Our finding may shed new light on Wolbachia as a possible agent causing hybrid dysfunction resulting from experimental crosses between chromosome races or subraces of P.sapporensis.