cGMP-dependent protein kinase I is involved in neurite outgrowth via a Rho effector, rhotekin, in Neuro2A neuroblastoma cells.
cGMP-dependent protein kinase I is involved in neurite outgrowth via a Rho effector, rhotekin, in Neuro2A neuroblastoma cells.
复制标题
在 Neuro2A 神经母细胞瘤细胞中，cGMP 依赖性蛋白激酶 I 通过 Rho 效应器 rhotekin 参与神经突生长。
DOI：
10.1016/j.bbrc.2012.03.143
复制
发表时间：
2012
影响因子：
3.1
通讯作者：
Keizo Yuasa
中科院分区：
文献类型：
--
作者：
Yayoi Onda;Yasushi Kawagoe;Keizo Yuasa
虽然cGMP/cGMP依赖的蛋白激酶（cGK）信号通路参与了神经突起生长的调控，但其机制尚不清楚。在这项研究中，我们确定了Rho效应，rhotekin，作为cGK-I相互作用蛋白。Rhotekin也是cGK-Iα的底物。在神经突延伸的Neuro 2A神经母细胞瘤细胞中，cGK-Iα和rhotekin共定位于质膜和延伸的神经突中，而cGMP处理导致rhotekin易位到细胞质中。此外，我们发现cGK-Iα和rhotekin协同抑制Rho诱导的神经突收缩。我们的研究结果表明，cGK-Iα与rhotekin相互作用并磷酸化rhotekin，从而有助于神经突生长调节。
Although the cGMP/cGMP-dependent protein kinase (cGK) signaling is involved in the regulation of neurite outgrowth, its mechanism remains to be clarified. In this study, we identified a Rho effector, rhotekin, as a cGK-I-interacting protein. Rhotekin was also a substrate for cGK-Iα. In neurite-extended Neuro2A neuroblastoma cells, cGK-Iα and rhotekin were colocalized in the plasma membrane and extended neurites, while treatment with cGMP resulted in translocation of rhotekin to the cytoplasm. In addition, we found that cGK-Iα and rhotekin synergistically suppressed Rho-induced neurite retraction. Our findings suggest that cGK-Iα interacts with and phosphorylates rhotekin, thereby contributing to neurite outgrowth regulation.