Variability in expression of a cell surface determinant on Candida albicans as evidenced by an agglutinating monoclonal antibody
Variability in expression of a cell surface determinant on Candida albicans as evidenced by an agglutinating monoclonal antibody
复制标题
凝集单克隆抗体证明白色念珠菌细胞表面决定簇表达的变异性
作者：
D. L. Brawner;J. Cutler
用一种凝集性免疫球蛋白M单克隆抗体研究了白色念珠菌一种表面免疫决定簇的表达。所检测的96株白色念珠菌（其中76%是近期临床分离株）都能够表达该抗原。热带念珠菌和光滑球拟酵母菌的菌株也产生这种抗原，但其他酵母菌种则不产生。如凝集反应和间接免疫荧光试验所示，白色念珠菌表面免疫决定簇的表达随生长情况而变化。用凝集素检测酵母细胞时，观察到三种反应模式。一般来说，对数生长早期的细胞比对数生长中期的细胞反应性弱。由凝集素反应性所确定的抗原表达也受生长培养基营养成分的影响，一般来说，肉汤培养的细胞通常比固体培养基上生长的细胞反应性更强。该抗原可通过1M热氯化钠从白色念珠菌细胞表面溶解下来。这些水溶性提取物能够结合抗体，并且当可溶性抗原在双向琼脂扩散试验中与单克隆抗体反应时，形成一条单一的沉淀线。与抗体混合时不发生凝集的酵母菌株的全细胞制剂和热氯化钠提取物也不从溶液中吸收凝集素。这些数据表明，白色念珠菌表面抗原的表达是一个动态过程，可能受多种环境因素的影响。单克隆抗体的使用可能有助于对念珠菌病期间呈现给宿主的表面抗原进行特性分析。
The expression of a surface immunodeterminant of Candida albicans was investigated with an agglutinating immunoglobulin M monoclonal antibody. The 96 strains of C. albicans tested, of which 76% were recent clinical isolates, were capable of expressing the antigen. The antigen was also produced by strains of Candida tropicalis and Torulopsis glabrata, but not by other yeast species. Expression of the surface immunodeterminant in C. albicans varied as a function of growth as indicated by agglutinin reactions and indirect immunofluorescence tests. When yeast cells were tested with the agglutinin, three patterns of reactivity were observed. In general, cells in the early logarithmic phase were less reactive than cells in the mid-logarithmic phase. Antigen expression, as determined by agglutinin reactivity, was also influenced by the nutritional composition of the growth medium, and in general, cells from broth cultures were usually more reactive than cells grown on solid media. The antigen was solubilized from the cell surface of C. albicans by hot 1 M NaCl. These water-soluble extracts were capable of binding antibody, and a single precipitin band formed when soluble antigen was reacted with the monoclonal antibody in an Ouchterlony double-diffusion test. Whole cell preparations and hot NaCl extracts from yeast strains which did not agglutinate when mixed with the antibody also did not absorb the agglutinin from solution. These data indicate that the expression of surface antigens on C. albicans is a dynamic process which may be influenced by a number of environmental factors. The use of monoclonal antibodies may allow characterization of surface antigens presented to the host during candidiasis.