QTL analysis of Fusarium head blight resistance using a high-density linkage map in barley
QTL analysis of Fusarium head blight resistance using a high-density linkage map in barley
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DOI：
10.1007/s00122-005-0102-4
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发表时间：
2005-11-01
影响因子：
5.4
通讯作者：
Takeda, K
中科院分区：
文献类型：
--
作者：
Hori, K;Kobayashi, T;Takeda, K
在俄罗斯 6（抗性）和 H.E.S. 杂交的一组重组自交 (RI) 系中评估了赤霉病 (FHB) 抗性。 4（易感），其中 FHB 耐药反应差异最大的之一。冈山大学5,000份大麦种质资源。通过“切穗试验”对田间种植的穗进行取样和接种。对亲本和 RI 品系的耐药反应分为十一个等级，从耐药 (0) 到易感 (10)。数量性状位点（QTL）分析检测到3个QTL：两个位于2H染色体长臂，另一个位于5H染色体短臂。位于 2H 染色体上的 QTL 与控制花序行型的 vrs1 基因座一致。 2H 染色体上的另一个 QTL 位于决定花序打开/关闭的闭花受精基因座（cly1 或 Cly2）附近。与抗病基因同源的抗性基因类似物（RGA）和表达序列标签（EST）标记被整合到高密度连锁图谱中。除了位于 cly1/Cly2 基因座附近的一个 RGA 标记 (FXLRRfor_XLRRrev170) 之外，大多数标记并不位于已识别的抗性 QTL 附近。对位于已识别 QTL 附近的 5 个 AFLP 标记进行测序，将其转换为序列标记位点 (STS) 标记。使用两个 AFLP-STS 标记和 vrs1 位点对每个 RI 品系进行基因分型表明，具有三个 Russia 6 QTL 等位基因的 RI 品系表现出与 Russia 6 相同水平的高 FHB 抗性反应。相反，具有三个易感等位基因的 RI 品系显示出与 H.E.S. 接近的反应。 4.因此，与QTL紧密连锁的标记可以有效地用于抗性选择。
Fusarium head blight (FHB) resistance was evaluated in a set of recombinant inbred (RI) lines from a cross between Russia 6 (resistant) and H.E.S. 4 (susceptible), which had one of the widest differences of FHB resistance reactions among ca. 5,000 barley germplasm accessions in Okayama University. Field-grown spikes were sampled and inoculated by the 'cut-spike test'. Resistance reactions on the parents and RI lines were scored by eleven grades, from resistant (0) to susceptible (10). Quantitative trait loci (QTL) analysis detected three QTL: two located on the long arm of chromosome 2H, and another on the short arm of chromosome 5H. A QTL located on chromosome 2H was coincident with the vrs1 locus, which governs inflorescence row type. The other QTL on chromosome 2H was positioned in the vicinity of cleistogamy locus (cly1 or Cly2) that determines inflorescence opening/closing. Resistant gene analog (RGA) and expressed sequence tag (EST) markers with homology for disease resistance genes were integrated into the high-density linkage map. Most of these markers were not localized near the identified resistance QTL, except for one RGA marker (FXLRRfor_XLRRrev170) localized in the vicinity of the cly1/Cly2 locus. Five AFLP markers localized in the vicinity of the identified QTL were sequenced to convert them into sequence tagged site (STS) markers. Genotyping of each RI line using two AFLP-STS markers and the vrs1 locus indicated that the RI lines with three Russia 6 QTL alleles exhibited the same level of high FHB resistance reactions as Russia 6. In contrast, RI lines with three susceptible alleles showed reactions close to H.E.S. 4. Therefore, the markers closely linked to the QTL can be efficiently used for the selection of resistance.