Analysis of the aerobactin and ferric hydroxamate uptake systems of Yersinia pestis
Analysis of the aerobactin and ferric hydroxamate uptake systems of Yersinia pestis
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DOI：
10.1099/mic.0.2006/004275-0
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发表时间：
2007-07-01
期刊：
影响因子：
2.8
通讯作者：
Fetherston, Jacqueline D.
中科院分区：
文献类型：
--
作者：
Forman, Stanislav;Nagiec, Michal J.;Fetherston, Jacqueline D.
鼠疫耶尔森氏菌基因组含有与大肠杆菌的需氧肌动蛋白受体（iutA）和生物合成基因（iucABCD）以及异羟肟酸铁摄取系统（fhuCDB）同源的基因。然而，iucA被移码突变破坏。大肠大肠杆菌中获得了携带克隆的Y.鼠疫菌的需氧肌动蛋白区域不能产生需氧肌动蛋白，但可以利用铁载体作为铁源。iucA移码突变的修复不允许在大肠杆菌或Y.鼠疫相比之下，Y.具有编码来自福氏志贺菌的iucABCD-iutA基因或pCoIV-K30的质粒的鼠疫菌株确实产生并分泌铁载体。此外，编码iucABCD-iutA位点而不编码Y. pestis特异性移码突变，也未能产生好氧菌素。鼠疫耶尔森鼠疫fhuCDB操纵子编码一系列异羟肟酸盐铁载体的ABC转运蛋白，能够补充一种在fhuC中插入转座子的大肠杆菌菌株，允许利用需氧菌素和铁色素。Y.鼠疫KIM 6是一种铁载体耶尔森氏杆菌素生产缺陷的菌株，能够使用铁色素和需氧杆菌素铁载体作为铁源。iutA或fhu操纵子的突变消除了KIM 6使用需氧菌素的能力。fhu操纵子中的突变，而不是iutA中的突变，影响了KIM 6使用铁色素的能力。这表明Y.鼠疫菌使用铁色素和需氧菌素，但失去了合成需氧菌素的能力。
Yersinia pestis genomes contain genes homologous to the aerobactin receptor (iutA) and biosynthetic genes (iucABCD) as well as the ferric hydroxamate uptake system (fhuCDB) of Escherichia coli. However, iucA is disrupted by a frameshift mutation. An E. coli strain carrying the cloned Y. pestis aerobactin region was unable to produce aerobactin, but could use the siderophore as an iron source. Repair of the frameshift mutation in iucA did not allow aerobactin production in E coli or Y. pestis. In contrast, a Y. pestis strain with a plasmid encoding the iucABCD-iutA genes from Shigella flexneri or pCoIV-K30 did produce and secrete the siderophore. In addition, Yersinia pseudo tuberculosis PB1, which encodes the iucABCD-iutA locus without the Y. pestis-specific frameshift mutation, also failed to produce aerobactin. The Y. pestis fhuCDB operon, encoding an ABC transporter for a range of hydroxamate siderophores, was able to complement a strain of E coli with a transposon insertion in fhuC, allowing utilization of aerobactin and ferrichrome. Y. pestis KIM6, a strain deficient in the production of the siderophore yersiniabactin, was able to use both the ferrichrome and the aerobactin siderophores as a source of iron. Mutations in iutA or the fhu operon abolished the ability of KIM6 to use aerobactin. Mutations in the fhu operon, but not in iutA, affected the ability of KIM6 to use ferrichrome. This demonstrates that Y. pestis uses both ferrichrome and aerobactin, but has lost the ability to synthesize aerobactin.