Compound A Induces Sister Chromatid Exchanges in Chinese Hamster Ovary Cells
Compound A Induces Sister Chromatid Exchanges in Chinese Hamster Ovary Cells
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化合物 A 诱导中国仓鼠卵巢细胞姐妹染色单体交换
DOI：
10.1097/00000542-199704000-00022
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发表时间：
1997
期刊：
影响因子：
8.8
通讯作者：
D. Gong
中科院分区：
文献类型：
--
作者：
E. Eger;M. Laster;R. Winegar;C. Han;D. Gong
背景：化合物A[CF2=C(CF3)OCH2F]是七氟烷[(CF3)2CHOCH2F]的降解产物，是乙烯基醚，可能是烷基化试剂。因此，它是一种潜在的遗传毒素。方法：测定代谢活化前后中国仓鼠卵巢细胞产生姐妹染色单体互换的能力。浓度为11~468ppm的化合物A作用2 h，在溴脱氧尿嘧啶核苷存在下继续孵育34 h，然后加入Colcemid使其停滞于中期。对细胞的编码玻片进行盲法检测，每种检测浓度计数50条染色体。结果：不加代谢激动剂的最低浓度(27ppm)的化合物A显著增加(P<；0.001)姐妹染色单体互换，增加化合物A的浓度可增加姐妹染色单体互换的发生率。代谢激活不会增加交换的发生率。结论：当七氟醚的浓度接近最低肺泡浓度时，化合物A在低流量系统中获得的浓度(27ppm)可增加姐妹染色单体交换。
Background: Compound A [CF2 = C(CF3)OCH2 F], a degradation product of sevoflurane [(CF3)2 CHOCH2 F], is a vinyl ether and may be an alkylating agent. Thus it is a potential genotoxin. Methods: The capacity of compound A to produce sister chromatid exchanges was measured in Chinese hamster ovary cells with and without metabolic activation. Concentrations of 11 to 468 ppm compound A were applied for 2 h, the Chinese hamster ovary cells were incubated for a further 34 h in the presence of bromodeoxyuridine, and then colcemid was added to produce arrest in metaphase. Coded slides of cells were examined blindly, and 50 chromosome spreads were counted for each test concentration. Results: The lowest concentration of compound A applied without metabolic activator (27 ppm) significantly increased (P < 0.001) sister chromatid exchanges, and increasing concentrations of compound A increased the incidence of exchanges. Metabolic activation did not increase the incidence of exchanges. Conclusions: Compound A increases sister chromatid exchanges at concentrations (27 ppm) obtained in low‐flow systems when sevoflurane is used at concentrations approximating minimum alveolar concentration.