Synthesis, Characterization, and Biological Evaluation of Nanostructured Hydroxyapatite with Different Dimensions.
Synthesis, Characterization, and Biological Evaluation of Nanostructured Hydroxyapatite with Different Dimensions.
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不同尺寸纳米结构羟基磷灰石的合成、表征及生物学评价
DOI：
10.3390/nano7020038
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发表时间：
2017-02-15
期刊：
影响因子：
--
通讯作者：
Yang X
中科院分区：
文献类型：
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作者：
Geng Z;Yuan Q;Zhuo X;Li Z;Cui Z;Zhu S;Liang Y;Liu Y;Bao H;Li X;Huo Q;Yang X
纳米羟基磷灰石(HA)是一种很有前途的替代骨中磷灰石的生物医学材料。此外，由于其良好的骨生物活性，纳米锶取代羟基磷灰石(SrHA)引起了人们的浓厚兴趣。然而，应该考虑这些纳米颗粒对细胞生物活性的尺寸效应。本研究采用水热法制备了不同尺寸、不同晶型的纳米HA和SrHA。用X射线衍射仪(X射线衍射仪)和傅立叶变换红外光谱仪(FT-IR)对样品进行了物相分析、晶化分析和化学成分分析。用场发射扫描电子显微镜(FE-SEM)和透射电子显微镜(TEM)观察了薄膜的形貌。通过电感耦合等离子体质谱(ICPMS)测定样品的离子释放谱来监测样品的降解行为。钙和锶的释放行为表明，降解速率与比表面积成正比，与结晶度成反比。MG63细胞的体外实验表明，长度大于100 nm的纳米棒在细胞增殖和分化方面都具有最好的生物学性能(与HA-1和SrHA-1相比P<；0.05；与HA-2相比P<；0.01)。此外，长径比较小的HA纳米颗粒比长径比较大的HA纳米颗粒具有更好的生物活性(P<；0.05)。本研究表明，纳米HA和SrHA具有细微的差异(包括尺寸、结晶、比表面积和降解率)，可以影响细胞的生长，从而可能对体内骨生长产生影响。这项工作提供了纳米HAS作为理想的生物相容性材料在未来临床应用中的作用的观点。
Nanosized hydroxyapatite (HA) is a promising candidate for a substitute for apatite in bone in biomedical applications. Furthermore, due to its excellent bone bioactivity, nanosized strontium-substituted HA (SrHA) has aroused intensive interest. However, the size effects of these nanoparticles on cellular bioactivity should be considered. In this study, nanosized HA and SrHA with different dimensions and crystallization were synthesized by hydrothermal methods. The phase, crystallization and chemical composition were analyzed by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR), respectively. The morphology was observed under field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM). The degradation behaviors of the samples were monitored by determining the ions release profile with inductively coupled plasma mass spectrometry (ICP-MS). The releasing behavior of Ca2+ and Sr2+ showed that the degradation rate was proportional to the specific surface area and inversely proportional to crystallization. The in vitro experiment evaluated by MG63 cells showed that SrHA nanorods with a length greater than 100 nm had the best biological performance both in cell proliferation and differentiation (* p < 0.05 compared with HA-1 and SrHA-1; * p < 0.01 compared with HA-2). In addition, HA nanoparticles with a lower aspect ratio had better bioactivity than higher ones (* p < 0.05). This study demonstrated that nanosized HA and SrHA with subtle differences (including dimensions, crystallization, specific surface area, and degradation rate) could affect the cellular growth and thus might have an impact on bone growth in vivo. This work provides a view of the role of nano-HAs as ideal biocompatible materials in future clinical applications.