DIAPHORINA CITRI (HEMIPTERA: PSYLLIDAE) INFECTION AND DISSEMINATION OF THE ENTOMOPATHOGENIC FUNGUS ISARIA FUMOSOROSEA (HYPOCREALES: CORDYCIPITACEAE) UNDER LABORATORY CONDITIONS
DIAPHORINA CITRI (HEMIPTERA: PSYLLIDAE) INFECTION AND DISSEMINATION OF THE ENTOMOPATHOGENIC FUNGUS ISARIA FUMOSOROSEA (HYPOCREALES: CORDYCIPITACEAE) UNDER LABORATORY CONDITIONS
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DOI：
10.1653/024.092.0413
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发表时间：
2009-12-01
影响因子：
1.4
通讯作者：
Rogers, Michael E.
中科院分区：
文献类型：
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作者：
Avery, Pasco B.;Hunter, Wayne B.;Rogers, Michael E.
采用离体叶片生物测定法测定了玫烟色棒束孢在柑桔木虱Diaphorina citri Kuwayama间的感染力和水平转移，其中将芽生孢子喷洒在柑桔叶节或黄色塑料标签（人工引诱剂表面）上。将四个叶切片或三个叶切片和一个黄色标签一起放置在皮氏培养皿室中。一至四个叶部分或黄色标签用I.烟色孢团（1.2 - 1.7 × 10（3）个芽生孢子/mm（2））。在处理物干燥后，将单个成虫木虱释放到每个室中。死亡率由I.在暴露于病原体后4.9 +/- 0.21- 6.1 +/- 0.37天观察到木虱成虫的玫烟色。定殖率高。在施用后第8天，在具有未处理的叶部分和一个处理的黄色标签的室中，玫烟色藓对成虫的诱导死亡率与在具有一个处理的叶部分的室中一样有效。在高湿度条件下，I.烟色孢菌芽生孢子容易在叶片表面产生菌丝，这对于确定成虫是否负责真菌的传播是有用的。在具有单个处理过的叶部分的室中，成虫与芽生孢子接触，并将这些芽生孢子移动到未处理的叶。当将木虱放入具有经处理的黄色标签的室中时，观察到木虱感染和随后真菌传播到未经处理的叶子的相同现象。I的使用。烟色菌接种的黄色标签具有作为木虱传播技术用于管理害虫种群的潜力。
The infectivity and horizontal transfer of Isaria fumosorosea Wize among Diaphorina citri Kuwayama was measured using a detached leaf bioassay in which blastospores were sprayed on citrus leaf sections or yellow plastic tags (artificial attractant surface). Four leaf sections or three leaf sections and one yellow tag were placed together in a Petri dish chamber. One to four of the leaf sections or the yellow tag was sprayed with I. fumosorosea (1.2 -1.7 x 10(3) blastospores/mm(2)). After treatments dried, a single adult psyllid was released into each chamber. Mortality due to I. fumosorosea for the adult psyllid was observed 4.9 +/- 0.21- 6.1 +/- 0.37 d following exposure to the pathogen. The rate of colonization by I. fumosorosea on adults in chambers with untreated leaf sections and one treated yellow tag was as effective in inducing mortality as in chambers with one treated leaf section at 8 days post application. Under high humidity, I. fumosorosea blastospores readily produced hyphae on the surface of leaves which was useful for determining if adults were responsible for transmission of the fungus. In chambers with a single treated leaf section, adults came into contact with blastospores and moved these around to the non-treated leaves. The same phenomenon, of psyllid infection and subsequent spreading of the fungus to non-treated leaves, was observed when psyllids were placed into chambers with a treated yellow tag. The use of I. fumosorosea inoculated yellow tags has potential as a psyllid dissemination technique for managing pest populations.