The effect of accelerated mineral trioxide aggregate on odontoblastic differentiation in dental pulp stem cell niches
The effect of accelerated mineral trioxide aggregate on odontoblastic differentiation in dental pulp stem cell niches
复制标题
DOI：
10.1111/iej.12747
复制
发表时间：
2018-07-01
影响因子：
5
通讯作者：
Kargul, B.
中科院分区：
文献类型：
--
作者：
Kulan, P.;Karabiyik, O.;Kargul, B.
目的探讨三氧化矿物聚合物（MTA）对人牙髓细胞龛（DPSC）增殖及向成牙本质细胞分化的影响。（WMTA; Dentsply塔尔萨牙科公司，约翰逊城，田纳西州，美国）与各种添加剂混合，所述添加剂包括蒸馏水、2.5%磷酸氢二钠（Na 2 HPO 4; Merck，达姆施塔特，德国）和5%氯化钙（CaCl 2; Merck）。从第三磨牙中提取的DPSC龛直接在培养基中的MTA上培养。通过3-（4，5-二甲基噻唑-2-基）-5-（3-羧基甲氧基苯基）-2-（4-磺基苯基）-2H-四唑鎓（MTS）测定评价细胞活力。采用实时聚合酶链反应分析法测定细胞生长和成牙本质细胞分化标志物（牙本质涎磷蛋白（DSPP）和1型胶原（COL 1））的表达。骨/牙源性分化的DPSC龛通过测量碱性磷酸酶活性（ALP）。使用von Kossa染色评估钙沉积。结果MTA与5%CaCl2和2.5%Na2HPO4混合后细胞活力最高（P
AimTo investigate the effect of accelerated-set mineral trioxide aggregate (MTA) on the proliferation and odontoblastic differentiation of human dental pulp cell niches (DPSC).MethodologyProRoot White MTA (WMTA; Dentsply Tulsa Dental, Johnson City, TN, USA) was mixed with various additives, which included distilled water, 2.5% disodium hydrogen phosphate (Na2HPO4; Merck, Darmstadt, Germany) and 5% calcium chloride (CaCl2; Merck). DPSC niches extracted from third molars were cultured directly on MTA in the culture medium. Cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4- sulphophenyl)-2H-tetrazolium (MTS) assay. Cell growth and expression of odontoblastic differentiation markers (dentine sialophosphoprotein (DSPP) and collagen type 1 (COL1)) were determined using Real-Time Polymerase Chain Reaction analysis. Osteo-/odontogenic differentiation of DPSC niches was evaluated by measurement of alkaline phosphatase activity (ALP). Calcium deposition was assessed using von Kossa staining. The results were analysed statistically using Mann-Whitney tests and Kruskal-Wallis tests.ResultsMTA mixed with 5% CaCl2 and 2.5% Na2HPO4 exhibited optimal cell viability (P