Pc4, a putative movement protein of Rice stripe virus, interacts with a type I DnaJ protein and a small Hsp of rice
Pc4, a putative movement protein of Rice stripe virus, interacts with a type I DnaJ protein and a small Hsp of rice
复制标题
Pc4 是水稻条纹病毒的一种推定运动蛋白，与 I 型 DnaJ 蛋白和水稻的小 Hsp 相互作用
DOI：
10.1007/s11262-008-0324-z
复制
发表时间：
2009-04-01
期刊：
影响因子：
1.6
通讯作者：
Wu, Zujian
中科院分区：
文献类型：
--
作者：
Lu, Lianming;Du, Zhenguo;Wu, Zujian
水稻条纹病毒（RSV）感染水稻并导致一些亚洲国家减产。本研究以 RSV 运动蛋白 pc4 为诱饵，通过基于 Gal4 的酵母双杂交系统筛选水稻 cDNA 文库。鉴定出许多阳性菌落，序列分析表明它们可能对应于十个独立的蛋白质。其中两个被选择并进一步表征。这两种蛋白分别是 J 蛋白和小 Hsp。使用免疫共沉淀证实了 Pc4 和两种蛋白质之间的相互作用。讨论了 pc4 与两种伴侣蛋白相互作用的发现的意义。
Rice stripe virus (RSV) infects rice and causes great yield reduction in some Asian countries. In this study, rice cDNA library was screened by a Gal4-based yeast two-hybrid system using pc4, a putative movement protein of RSV, as the bait. A number of positive colonies were identified and sequence analysis revealed that they might correspond to ten independent proteins. Two of them were selected and further characterized. The two proteins were a J protein and a small Hsp, respectively. Interactions between Pc4 and the two proteins were confirmed using coimmunoprecipitation. Implications of the findings that pc4 interacted with two chaperone proteins were discussed.