IDENTIFICATION OF A NOVEL ISOFORM OF THE RETINOIC ACID RECEPTOR-GAMMA EXPRESSED IN THE MOUSE EMBRYO
IDENTIFICATION OF A NOVEL ISOFORM OF THE RETINOIC ACID RECEPTOR-GAMMA EXPRESSED IN THE MOUSE EMBRYO
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DOI：
10.1128/mcb.10.5.2335
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发表时间：
1990-05-01
影响因子：
5.3
通讯作者：
VARMUZA, S
中科院分区：
文献类型：
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作者：
GIGUERE, V;SHAGO, M;VARMUZA, S
维甲酸对发育过程有深远的影响。它被认为是发育中的鸡肢芽和再生的两栖动物肢胚的形态原，并已被证明对包括人类在内的哺乳动物具有强大的致畸作用。最近，三种特异性视黄酸受体（RARs）， rar . α。度量。和。gamma。，被鉴定并证明是类固醇受体超家族的成员。我们报告了一种新的rar伽玛的鉴定。同种型,mRAR.gamma。B，它不同于先前描述的小鼠RAR.gamma。在氨基端。此外，我们还证明了RAR.gamma。同种异构体在妊娠中期在已知受维甲酸给药对妊娠小鼠不利影响的结构中表达最多。因此，在小鼠胚胎中表达了多个RAR亚型，其中每个亚型都可能作为哺乳动物发育的调节剂发挥独特或组合作用。
Retinoic acid is known to have profound effects on developmental processes. It has been implicated as a putative morphogen in the developing chick limb bud and regenerating amphibian limb blastema and has been demonstrated to have powerful teratogenic effects in mammals, including humans. Recently, three specific retinoic acid receptors (RARs), RAR.alpha., -.beta., and .gamma., were identified and shown to be members of the steriod receptor superfamily. We report the identification of a novel RAR.gamma. isoform, mRAR.gamma.B, which differs from the previously described mouse RAR.gamma. at its amino terminus. In addition, we show that both RAR.gamma. isoforms are expressed maximally at midgestation in structures known to be affected adversely by retinoic acid administration to pregnant mice. Multiply RAR isoforms, each of which may play a unique or combinatorial role as a regulator of mammalian development, are thus expressed in the mouse embryo.