Streptomycin production by Streptomyces griseus can be modulated by a mechanism not associated with change in the adpA component of the A-factor cascade
Streptomycin production by Streptomyces griseus can be modulated by a mechanism not associated with change in the adpA component of the A-factor cascade
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DOI：
10.1007/s10529-006-9216-2
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发表时间：
2007-01-01
影响因子：
2.7
通讯作者：
McDowall, Kenneth J.
中科院分区：
文献类型：
--
作者：
Hong, Bin;Phornphisutthimas, Somkiat;McDowall, Kenneth J.
在天蓝色链霉菌中，全反式维甲酸是放线菌素簇调节基因actII-ORF4的转录激活子。在以前的工作中，我们证明了天蓝色链球菌ATRA在体外与链霉素簇调节因子灰链霉菌STRR的启动子结合。我们发现灰色链霉菌携带ATRA的单一同源物，而天蓝色链球菌ATRA在灰色链霉菌中的表达导致链霉素的产生和STRR的mRNA水平以及链霉素生物合成的基因水平依赖于DNA结合而减少。然而，这对adpA的mRNA水平没有影响，adpA是到目前为止唯一被鉴定为STRR的转录因子。AdpA基因由ARPA直接调节，ARPA是γ-丁内酯信号分子A因子的受体蛋白。因此，据我们所知，我们的结果提供了第一个体内证据，表明A因子-ARPA-AdpA-STRR调控级联只代表了链霉素生物合成调控的全部复杂性的一部分。讨论了我们的发现在生物技术方面的潜在应用。
In Streptomyces coelicolor, AtrA is an activator of transcription of the actinorhodin cluster-situated regulator gene actII-ORF4. In previous work, we showed that S. coelicolor AtrA binds in vitro to the promoter of S. griseus strR, the streptomycin cluster-situated regulator. We show here that S. griseus carries a single close homologue of atrA and that expression of S. coelicolor AtrA in S. griseus causes a DNA binding-dependent reduction in streptomycin production and in the mRNA levels of strR and genes of streptomycin biosynthesis. However, there is no effect on the level of the mRNA of adpA, which is the only transcription factor that has so far been characterised for strR. The adpA gene is directly regulated by ArpA, the receptor protein for the gamma-butyrolactone signalling molecule A-factor. Therefore, to our knowledge, our results provide the first in vivo evidence that A-factor-ArpA-AdpA-StrR regulatory cascade represents only part of the full complexity of regulation of streptomycin biosynthesis in S. griseus. The potential biotechnological application of our findings is discussed.