Differential expression of cell activation markers after stimulation of resting human B lymphocytes.
Differential expression of cell activation markers after stimulation of resting human B lymphocytes.
复制标题
刺激静息人 B 淋巴细胞后细胞激活标记物的差异表达。
DOI：
10.4049/jimmunol.132.6.2857
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发表时间：
1984
影响因子：
4.4
通讯作者：
A. Fauci
中科院分区：
文献类型：
--
作者：
J. Kehrl;A. Muraguchi;A. Fauci
抗原与表面IG受体的相互作用启动了一个复杂的过程，通过该过程，静息B淋巴细胞被激活。在这项研究中，小的静息B淋巴细胞通过扁桃体B淋巴细胞的逆流淘析分离，并用一系列浓度的抗mu和B细胞生长因子（BCGF）刺激。使用单克隆抗体4 F2和5 E9分析了已知静息B淋巴细胞中不存在但存在于活化细胞上的两种糖蛋白的后续表达。由4F 2单克隆抗体识别的抗原在细胞周期的G1期早期表达，并与原始细胞转化相关;这通过用足以激活细胞但不增殖的低浓度抗mu刺激静息B细胞来证明。除非用较高浓度的抗mu刺激B细胞或将BCGF加入低浓度的抗mu中，否则不存在5E 9定义的抗原。向培养物中加入羟基脲阻断了刺激的B细胞进入S期，但不干扰4F 2或5E 9的表达。因此，单克隆抗体4F 2识别G1早期的细胞，而5E 9识别G1晚期的细胞。通过碘化丙啶和4F 2或5E 9的双重免疫荧光染色，所有处于S、G2和M期的细胞均显示为4F 2和5E 9阳性。单克隆抗体4F 2和5E 9可用于区分活化的人B淋巴细胞和静息的人B淋巴细胞，并描述活化过程中的顺序步骤。
The interaction of antigen with surface Ig receptors initiates a complex process by which resting B lymphocytes are activated. In this study, small resting B lymphocytes were isolated by countercurrent elutriation of tonsillar B lymphocytes and stimulated with a range of concentrations of anti-mu and B cell growth factor (BCGF). The subsequent expression of two glycoproteins known to be absent from resting B lymphocytes but present on activated cells were analyzed with the monoclonal antibodies 4F2 and 5E9. The antigen recognized by the 4F2 monoclonal was expressed early in the G1 phase of the cell cycle and correlated with blast transformation; this was demonstrated by stimulating resting B cells with a low concentration of anti-mu sufficient for cellular activation but not proliferation. The 5E9-defined antigen was not present unless the B cells were stimulated with a higher concentration of anti-mu or a BCGF was added to the low concentration of anti-mu. The addition of hydroxyurea to culture blocked the entrance of stimulated B cells into S phase but did not interfere with the expression of either 4F2 or 5E9. Thus, whereas the monoclonal antibody 4F2 recognized cells in early G1, 5E9 recognized cells in late G1. By double immunofluorescence staining with propidium iodide and either 4F2 or 5E9, all cells in S, G2, and M phases were demonstrated to be both 4F2- and 5E9-positive. The monoclonal antibodies 4F2 and 5E9 can be used to distinguish an activated from a resting human B lymphocyte and to delineate sequential steps in the activation process.