Scrapie-like encephalopathy in a greater kudu (Tragelaphus strepsiceros) which had not been fed ruminant-derived protein
Scrapie-like encephalopathy in a greater kudu (Tragelaphus strepsiceros) which had not been fed ruminant-derived protein
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未饲喂反刍动物来源蛋白质的大捻角羚 (Tragelaphus strepsiceros) 出现痒病样脑病
DOI：
10.1136/vr.130.17.365
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发表时间：
1992
影响因子：
2.2
通讯作者：
J. Wilesmith
中科院分区：
文献类型：
--
作者：
J. Kirkwood;G. Wells;A. Cunningham;S. Jackson;A. Scott;M. Dawson;J. Wilesmith
15个月前，一只19个月大的大银鱼因海绵状脑病死亡，在患上严重的共济失调和抑郁症后，需要安乐死，显然是突然发作。尸检未发现肉眼可见异常，但脑部和脊髓节段的组织病理学检查可见抓痒样海绵状脑脊髓病。用蛋白水解酶K处理的脑干组织洗涤剂提取物的负染电子显微镜观察到与瘙痒病相关的纤维，用兔抗小鼠PrP抗血清从家牛海绵状脑病的脑组织中检测到一条与异常PrP(PrP)相似的25~28 kDa条带。这只动物是在法定禁止在反刍动物饲料中加入反刍动物衍生蛋白的九个月后出生的，由于没有明显的其他可能的疾病来源，似乎感染是从大坝获得的。
A 19-month-old greater kudu (Tragelaphus strepsiceros), whose dam had died 15 months earlier with spongiform encephalopathy, required euthanasia after developing severe ataxia and depression with an apparently sudden onset. No macroscopic abnormalities were detected on post mortem examination but a scrapie-like spongiform encephalomyelopathy was apparent on histopathological examination of brain and segments of spinal cord. Negative stain electron microscopy of proteinase K-treated detergent extracts of tissue from the brain stem revealed the presence of scrapie associated fibrils, and a 25 to 28 kDa band comparable with that identified as abnormal PrP (prion protein) from the brains of domestic cattle with spongiform encephalopathy was detected using rabbit antiserum raised against mouse PrP. The animal was born nine months after the statutory ban on the inclusion of ruminant-derived protein in ruminant feeds and, as no other possible sources of the disease were apparent, it appears likely that the infection was acquired from the dam.