Evidence for sequential expression of multiple AMP deaminase isoforms during skeletal muscle development.
Evidence for sequential expression of multiple AMP deaminase isoforms during skeletal muscle development.
复制标题
骨骼肌发育过程中多种 AMP 脱氨酶亚型顺序表达的证据。
DOI：
10.1073/pnas.84.8.2345
复制
发表时间：
1987
影响因子：
11.1
通讯作者：
Holmes,EW
中科院分区：
文献类型：
--
作者：
Marquetant,R;Desai,NM;Sabina,RL;Holmes,EW
AMP脱氨酶(肌腺苷脱氨酶；EC 3.5.4.6)是骨骼肌肌原纤维的组成部分，在骨骼肌组织的能量代谢中起着重要作用。我们在此报告了大鼠骨骼肌发育过程中三种AMP脱氨酶亚型的鉴定。胚胎亚型在怀孕7至14天的大鼠发育中的后肢中表达。这种异构体并不是骨骼肌或胚胎所特有的，因为它在围产期和成年大鼠的许多非肌肉组织中也有表达。一种仅限于骨骼肌的围产期AMP脱氨酶亚型在出生前4-6天产生，并在出生后2-3周持续存在。成年的骨骼肌特异的AMP脱氨酶在出生时出现，在出生后3周达到最高水平。我们从这些研究中得出结论，在从胚胎骨骼肌到成年骨骼肌的转变过程中，存在一个发育控制程序，导致AMP脱氨酶亚型的顺序表达。
AMP deaminase (myoadenylate deaminase; EC 3.5.4.6) is an integral part of the myofibril in skeletal muscle, and this enzyme plays an important role in energy metabolism in this tissue. We report here the identification of three AMP deaminase isoforms during skeletal muscle development in the rat. An embryonic isoform is expressed in the developing hindlimb of the rat between 7 and 14 days of gestation. This isoform is not unique to skeletal muscle or the embryo as it is also expressed in many nonmuscle tissues of the perinatal and adult rat. A perinatal isoform of AMP deaminase that is restricted to skeletal muscle is produced 4-6 days before birth and persists for 2-3 weeks of postnatal life. An adult, skeletal muscle-specific isoform of AMP deaminase appears at birth and reaches maximal levels after 3 weeks of postnatal development. We conclude from these studies there is a developmentally controlled program that leads to the sequential expression of AMP deaminase isoforms during the transition from embryonic to adult skeletal muscle.