Electrospun three-dimensional hyaluronic acid nanofibrous scaffolds
Electrospun three-dimensional hyaluronic acid nanofibrous scaffolds
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DOI：
10.1016/j.biomaterials.2006.02.037
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发表时间：
2006-07-01
期刊：
影响因子：
14
通讯作者：
Rafailovich, MH
中科院分区：
文献类型：
--
作者：
Ji, Y;Ghosh, K;Rafailovich, MH
采用静电纺丝技术成功制备了三维透明质酸（HA）纳米纤维支架，模拟了天然细胞外基质（ECM）的结构。合成了硫代羟基透明质酸衍生物3,3′-二硫代丙二肼修饰羟基透明质酸（HA- dtph），并通过电纺丝制备了三维纳米纤维支架。为了促进静电纺丝过程中纤维的形成，将聚环氧乙烷（PEO）以1:1的最佳质量比加入到HA-DTPH水溶液中。电纺丝HA-DTPH/PEO共混支架随后通过聚乙二醇-二丙烯酸酯（PEGDA）介导的共轭加成交联。然后用去离子水提取PEO，得到电纺HA-DTPH纳米纤维支架。将NIH 3T3成纤维细胞体外接种于纤维连接蛋白吸附的HA-DTPH纳米纤维支架上24 h。荧光显微镜和激光扫描共聚焦显微镜显示，3T3成纤维细胞附着在支架上并扩散，在支架内表现出扩展的树突形态，这表明HA-DTPH纳米纤维支架在细胞包封和组织再生方面的潜在应用。(c) 2006 Elsevier Ltd.版权所有。
A three-dimensional (3D) hyaluronic acid (HA) nanofibrous scaffold was successfully fabricated to mimic the architecture of natural extracelluar matrix (ECM) based on electrospinning. Thiolated HA derivative, 3,3'-dithiobis(propanoic dihydrazide)-modified HA (HA-DTPH), was synthesized and electrospun to form 3D nanofibrous scaffolds. In order to facilitate the fiber formation during electrospinning, Poly (ethylene oxide) (PEO) was added into the aqueous solution of HA-DTPH at an optimal weight ratio of 1:1. The electrospun HA-DTPH/PEO blend scaffold was subsequently cross-linked through poly (ethylene glycol)-diacrylate (PEGDA) mediated conjugate addition. PEO was then extracted in DI water to obtain an electrospun HA-DTPH nanofibrous scaffold. NIH 3T3 fibroblasts were seeded on fibronectin-adsorbed HA-DTPH nanofibrous scaffolds for 24 h in vitro. Fluorescence microscopy and laser scanning confocal microscopy revealed that the 3T3 fibroblasts attached to the scaffold and spread, demonstrating an extended dendritic morphology within the scaffold, which suggests potential applications of HA-DTPH nanofibrous scaffolds in cell encapsulation and tissue regeneration. (c) 2006 Elsevier Ltd. All rights reserved.