Expression of adhesion molecules and fibronectin of activated peritoneal surface with Lipopolysaccharide (LPS) analyzed with Immuno SEM
Expression of adhesion molecules and fibronectin of activated peritoneal surface with Lipopolysaccharide (LPS) analyzed with Immuno SEM
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DOI：
10.1016/s0940-9602(01)80179-5
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发表时间：
2001-07-01
影响因子：
2.2
通讯作者：
Sasaki, K
中科院分区：
文献类型：
--
作者：
Liang, Y;Jyoukura, K;Sasaki, K
为了揭示与腹膜防御机制相关的细胞间相互作用，用脂多糖（LPS）刺激腹膜，并使用免疫SEM（扫描电子显微镜）进行三维、超微结构和免疫组织化学分析。活化的肝腹膜表面显示出大量带有粘附分子ICAM-1和VCAM-1的微绒毛。它们仅限于绒毛，LPS 浓度达到峰值为 1.5 微克/克体重。随着 LPS 的增加，细丝出现适度，并在微绒毛之间交织。这些链不表达 ICAM-1 或 VCAM-1，而是表达纤连蛋白。当 LPS 值（2.5 杯）以上时，白细胞开始粘附到腹膜表面。这些结果表明，腹膜表面通过粘附分子和纤连蛋白为细胞间相互作用提供了防御层。
To disclose cell-to-cell interaction associated with the defensive mechanism of the peritoneum, the peritoneum was stimulated with lipopolysaccharide (LPS) and analyzed three-dimensionally, ultrastructurally, and immunohistochemically with immunoSEM (scanning electron microscopy). The activated hepatic peritoneal surface demonstrated numerous microvilli with the adhesion molecules ICAM-1 and VCAM-1. They were restricted to villi and peaked at 1.5 mug/g body weight of LPS. Delicate strands appeared moderately and were interwoven among microvilli with increasing LPS. These strands did not express ICAM-1 or VCAM-1, but fibronectin. Leukocytes began to adhere to the peritoneal surface above die value of LPS (2.5 mug). These results suggest that the peritoneal surface gives a defensive sheet for cell-to-cell interaction through adhesion molecules and fibronectin.