Females and males exhibit similar functional, mechanical, and morphological outcomes in a rat model of posttraumatic elbow contracture.
Females and males exhibit similar functional, mechanical, and morphological outcomes in a rat model of posttraumatic elbow contracture.
复制标题
在创伤后肘关节挛缩的大鼠模型中，雌性和雄性表现出相似的功能、机械和形态学结局。
DOI：
10.1002/jor.24918
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发表时间：
2021-09
期刊：
影响因子：
--
通讯作者：
Lake SP
中科院分区：
文献类型：
--
作者：
Reiter AJ;Schott HR;Castile RM;Cannon PC;Havlioglu N;Chamberlain AM;Lake SP
创伤后关节痉挛(PTJC)是一种以损伤后关节活动丧失为特征的衰弱状态。先前在肘部PTJC大鼠模型中的工作仅在雄性动物中调查了疾病的病因、进展和恢复；这项研究探索了基于性别的差异。对大鼠肘部进行单侧前囊切开和外侧副韧带切断，然后分别进行42天的制动和42天的自由活动。在整个自由活动期间收集握力和步态，同时进行关节力学测试、微型计算机断层扫描和组织学分析。总体而言，在功能、机械和形态结果上，性别之间几乎没有差异，在雄性和雌性动物中，PTJC类似地使人虚弱。握力和步态的功能测量表明，虽然性别之间存在一些基线差异，但创伤会产生类似的缺陷，与对照动物相比，长期而言仍有显著不同。同样，由于受伤，雄性和雌性动物的关节活动范围都显著减少。异位钙化(EC)，这是以前没有被评估的这个损伤模型，存在于所有肢体的外侧。损伤导致EC体积增加，但不改变骨密度，无论性别。此外，前囊膜的组织学分析显示，炎症和厚度在性别之间有微小的差异，但在其他组织学参数上没有差异。定量了解与这种损伤模型相关的性别差异将有助于为未来旨在减少或预防肘部PTJC的治疗提供参考。
Post-traumatic joint contracture (PTJC) is a debilitating condition characterized by loss of joint motion following injury. Previous work in a rat model of elbow PTJC investigated disease etiology, progression and recovery in only male animals; this study explored sex-based differences. Rat elbows were subjected to a unilateral anterior capsulotomy and lateral collateral ligament transection followed by 42 days of immobilization and 42 days of free mobilization. Grip strength and gait were collected throughout the free mobilization period while joint mechanical testing, micro-computed tomography and histological analysis were performed post-mortem. Overall, few differences were seen between sexes in functional, mechanical and morphological outcomes with PTJC being similarly debilitating in male and female animals. Functional measures of grip strength and gait showed that, while some baseline differences existed between sexes, traumatic injury produced similar deficits that remained significantly different long-term when compared to control animals. Similarly, male and female animals both had significant reductions in joint range of motion due to injury. Ectopic calcification (EC), which had not been previously evaluated in this injury model, was present in all limbs on the lateral side. Injury caused increased EC volume but did not alter mineral density regardless of sex. Furthermore, histological analysis of the anterior capsule showed minor differences between sexes for inflammation and thickness but not for other histological parameters. A quantitative understanding of sex-based differences associated with this injury model will help inform future therapeutics aimed at reducing or preventing elbow PTJC.