The role of three genetically distinct collagen types in endochondral ossification and calcification of cartilage
The role of three genetically distinct collagen types in endochondral ossification and calcification of cartilage
复制标题
三种遗传上不同的胶原蛋白类型在软骨内骨化和钙化中的作用
DOI：
--
复制
发表时间：
1977
期刊：
影响因子：
--
通讯作者：
H. Mark
中科院分区：
文献类型：
--
作者：
K. Mark;H. Mark
已经评估了三种基因上不同的胶原蛋白类型在软骨内骨形成和软骨钙化和吸收中的作用。我们利用针对I型、II型和III型胶原蛋白的抗体，在鸡胫骨胚胎中证实，软骨内骨形成始于III型胶原蛋白侵入骨髓源性细胞，沉积在增生性软骨细胞的腔隙中。随后是I型胶原的沉积，这是软骨内类骨的胶原成分。在发育后期，软骨内样骨出现于表面完整的增生性软骨细胞腔隙的骺端生长板内；这表明后者可能有助于类骨I型胶原的合成。胶原蛋白类型的免疫组织学染色和平行切片上磷酸钙的von Kossa染色表明，I型和II型胶原基质是钙化的底物。在未钙化和钙化软骨（含II型胶原）的支架上均发现软骨内骨（含I型胶原），表明软骨内类骨和底层软骨的钙化是独立发生的。4周龄鸡软骨内松质骨的针状体含有钙化的II型胶原蛋白核心。
The role of three genetically distinct collagen types in the formation of endochondral bone and in calcification and resorption of cartilage has been assessed. Using antibodies specific to types I, II and III collagen we have demonstrated in the embryonic chick tibia that endochondral bone formation began with deposition of type III collagen in lacunae of hypertropic chondrocytes by invading bone-marrow-derived cells. This was followed by the deposition of type I collagen, which is the collagenous constituent of endochondral osteoid. At later stages of development endochondral osteoid was found in the epiphysial growth plate in apparently intact lacunae of hypertrophic chondrocytes; this indicated that the latter might contribute to the synthesis of osteoid type I collagen. Immuno-histological staining for collagen types, and von Kossa staining for calcium phosphate on parallel sections, demonstrated that type I and type II collagen matrices were substrates for calcification. Endochondral bone (with type I collagen) was found on scaffolding of both uncalcified and calcified cartilage (with type II collagen), indicating that calcification of endochondral osteoid and of the underlying cartilage occurred independentyl. Spicules of endochondral cancellous bone of a four-week-old chick contained a core of calcified type II collagen.