Gender-associated gene expression in two related strains of Schistosoma japonicum
Gender-associated gene expression in two related strains of Schistosoma japonicum
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DOI：
10.1016/j.molbiopara.2004.03.014
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发表时间：
2004-08-01
影响因子：
1.5
通讯作者：
Hoffmann, KF
中科院分区：
文献类型：
--
作者：
Fitzpatrick, JM;Johansen, MV;Hoffmann, KF
宿主针对性成熟的日本血吸虫雌虫产下的卵的炎症反应，在感染过程中引发病变形成和相关的临床病理。为了鉴定与产卵相关的寄生虫基因转录本，并表征两种相关中国菌株性成熟成虫基因表达谱，利用来自3个寄生虫发育阶段的457条ESTs构建了日本血吸虫cDNA微阵列。在安徽成虫中鉴定出22个雌性相关基因转录本和8个雄性相关基因转录本，在浙江成虫中鉴定出21个雌性相关基因转录本和7个雄性相关基因转录本。RT-PCR分析、原位酶定位研究和酶分析证实了cDNA微阵列的结果，重要的是，提供了以前在血吸虫偶联生物学中未被重视的信息。具体来说，我们的新发现包括女性特异性基因的表达，这些基因被认为与血红蛋白消化和蛋壳形成有关，包括细胞外超氧化物歧化酶、两种富含组氨酸的蛋白质、一种大型血脑屏障氨基酸转运蛋白和两种酪氨酸酶同源物。相比之下，参与机械支持（肌动蛋白）、细胞骨架基础结构（如动力蛋白轻链3和肌球蛋白调节轻链）和被膜生物学（如TM4SF和Sj25）的转录本在成年雄性血吸虫中更有代表性。总之，这些数据建立了成虫劳动分工的转录基础，并扩大了日本血吸虫性别相关基因的新转录本清单，这些转录本可能被认为是改进干预策略的目标。(C) 2004 Elsevier B.V.版权所有
Host inflammatory responses directed against eggs laid by sexually-mature Schistosoma japonicum female worms instigate lesion formation and associated clinical pathologies during infection. To identify parasite gene transcripts that associate with egg production and to characterise sexually-mature adult gene expression profiles of two related Chinese strains, S. japonicum cDNA microarrays were fabricated using 457 ESTs originating from three parasite developmental stages. Twenty-two female-associated and 8 male-associated gene transcripts were identified in the adult Anhui strain whereas 21 female-associated and 7 male-associated gene transcripts were revealed in the adult Zhejiang strain. RT-PCR analysis, in situ enzyme localisation studies and enzymatic assays confirmed the cDNA microarray results, and importantly, provided information previously unappreciated in schistosome conjugal biology. Specifically, our novel findings include the female-specific expression of genes putatively involved in haemoglobin digestion and eggshell formation including extracellular superoxide dismutase, two histidine-rich proteins, a large blood-brain barrier amino acid transporter and two tyrosinase orthologues. In contrast, transcripts involved in mechanical support (actin), cytoskeletal infrastructure (e.g. dynein light chain 3 and myosin regulatory light chain) and tegumental biology (e.g. TM4SF and Sj25) were more highly represented in adult male schistosomes. Together these data establish a transcriptional basis for adult schistosome labour division and expands the list of novel S. japonicum gender-associated gene transcripts that may be considered targets for improved intervention strategies. (C) 2004 Elsevier B.V. All rights reserved.